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10.3892/mmr.2015.3991 http://scihub22266oqcxt.onion/10.3892/mmr.2015.3991 C4526098!4526098 !26126577     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 286.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 286.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 286.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 286.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26126577 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mol+Med+Rep 2015 ; 12 (3 ): 4079-4088 Nephropedia Template TP {{tp|p=26126577 |t=2015. Protection of rat intestinal epithelial cells from ischemia/reperfusion injury by (D-Ala2, D-Leu5)-enkephalin through inhibition of the MKK7-JNK signaling pathway |pdf=|usr=}}{{26126577 }} gab.com Text Protection of rat intestinal epithelial cells from ischemia/reperfusion injury by (D-Ala2, D-Leu5)-enkephalin through inhibition of the MKK7-JNK signaling pathway JO: Mol Med Rep http://www.kidney.de/pget.php?&tw=1&pmid=26126577 #FOAvip Previous studies have demonstrated that (D?Ala2, D?Leu5)?enkephalin (DADLE) protects rats from hepatic ischemia/reperfusion (I/R) injury. In the present study, DADLE was also observed to alleviate IR?induced intestinal epithelial cell injury in rats by inhibiting mitogen?activated protein kinase kinase 7 (MKK7)?c?Jun N?terminal kinase (JNK) pathway signaling. To investigate the protective effect of DADLE on hypoxia/reoxygenation injury in rat intestinal epithelial cells, rat intestinal epithelial cells were treated with different concentrations of DADLE, following which the cell survival rate was determined using a tetrazolium (MTT) colorimetric assay, and apoptosis was determined using flow cytometry. To confirm whether the protective effect of DADLE was due to its effect on MKK7?JNK signaling, the phosphorylation levels of MKK7 and JNK were analyzed using western blot analysis following treatment with different concentrations of DADLE. The results demonstrated that, following treatment with DADLE, the survival rate of the rat intestinal cells subjected to I/R?induced injury increased significantly and the apoptotic rate decreased in a concentration?dependent manner. In addition, the levels of phosphorylated MKK7 and JNK decreased in a concentration?dependent manner following treatment with DADLE. Silencing the gene expression of MKK7 using small interfering RNA prior to DADLE treatment resulted in a reduction in the protective effects of DADLE on the rat intestinal epithelial cells subjected to I/R injury. Collectively, the results of the present study demonstrated that the protective effects of DADLE in I/R injury in rat intestinal cells occurred through inhibition of the MKK7?JNK pathway. Twit Text FOAVip Protection of rat intestinal epithelial cells from ischemia/reperfusion injury by (D-Ala2, D-Leu5)-enkephalin through inhibition of the MKK7-JNK signaling pathway ????Mol Med Rep http://www.kidney.de/pget.php?&tw=1&pmid=26126577 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26126577 #FOAvip English Wikipedia <ref>{{Cite pmid|26126577 }}</ref> Protection of rat intestinal epithelial cells from ischemia/reperfusion injury by (D-Ala2, D-Leu5)-enkephalin through inhibition of the MKK7-JNK signaling pathway #MMPMID26126577 Wang Z ; Tang B ; Tang F ; Li Y ; Zhang G ; Zhong L ; Dong C ; He S Mol Med Rep 2015[Sep]; 12 (3 ): 4079-4088 PMID26126577 show ga Previous studies have demonstrated that (D?Ala2, D?Leu5)?enkephalin (DADLE) protects rats from hepatic ischemia/reperfusion (I/R) injury. In the present study, DADLE was also observed to alleviate IR?induced intestinal epithelial cell injury in rats by inhibiting mitogen?activated protein kinase kinase 7 (MKK7)?c?Jun N?terminal kinase (JNK) pathway signaling. To investigate the protective effect of DADLE on hypoxia/reoxygenation injury in rat intestinal epithelial cells, rat intestinal epithelial cells were treated with different concentrations of DADLE, following which the cell survival rate was determined using a tetrazolium (MTT) colorimetric assay, and apoptosis was determined using flow cytometry. To confirm whether the protective effect of DADLE was due to its effect on MKK7?JNK signaling, the phosphorylation levels of MKK7 and JNK were analyzed using western blot analysis following treatment with different concentrations of DADLE. The results demonstrated that, following treatment with DADLE, the survival rate of the rat intestinal cells subjected to I/R?induced injury increased significantly and the apoptotic rate decreased in a concentration?dependent manner. In addition, the levels of phosphorylated MKK7 and JNK decreased in a concentration?dependent manner following treatment with DADLE. Silencing the gene expression of MKK7 using small interfering RNA prior to DADLE treatment resulted in a reduction in the protective effects of DADLE on the rat intestinal epithelial cells subjected to I/R injury. Collectively, the results of the present study demonstrated that the protective effects of DADLE in I/R injury in rat intestinal cells occurred through inhibition of the MKK7?JNK pathway. |Animals [MESH] |Apoptosis Regulatory Proteins/metabolism [MESH] |Cell Line [MESH] |Cell Proliferation [MESH] |Cell Survival/drug effects [MESH] |Disease Models, Animal [MESH] |Enkephalin, Leucine-2-Alanine/*pharmacology [MESH] |Epithelial Cells/cytology/drug effects/metabolism [MESH] |Female [MESH] |G1 Phase Cell Cycle Checkpoints/drug effects [MESH] |Intestines/cytology [MESH] |JNK Mitogen-Activated Protein Kinases/*metabolism [MESH] |MAP Kinase Kinase 7/antagonists & inhibitors/genetics/*metabolism [MESH] |MAP Kinase Signaling System/*drug effects [MESH] |Male [MESH] |Neuroprotective Agents/*pharmacology [MESH] |Phosphorylation/drug effects [MESH] |RNA Interference [MESH] |RNA, Small Interfering/metabolism [MESH] |Rats [MESH] |Rats, Sprague-Dawley [MESH]Protection of rat intestinal epithelial cells from ischemia/reperfusio(epmc).pdf DeepDyve Pubget Overpricing