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10.3892/mmr.2015.3842 http://scihub22266oqcxt.onion/10.3892/mmr.2015.3842 C4526069!4526069 !26017616     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26017616 &cmd=llinks): Failed to open stream: HTTP request failed! 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Inhibition of liver fibrosis using vitamin A-coupled liposomes to deliver matrix metalloproteinase-2 siRNA in vitro |pdf=|usr=}}{{26017616 }} gab.com Text Inhibition of liver fibrosis using vitamin A-coupled liposomes to deliver matrix metalloproteinase-2 siRNA in vitro JO: Mol Med Rep http://www.kidney.de/pget.php?&tw=1&pmid=26017616 #FOAvip Hepatic fibrosis is a common form of wound healing in response to chronic liver injuries and can lead to more serious complications, including mortality. It is well?established that hepatic stellate cells (HSCs) are central mediators of hepatic fibrosis, and matrix metalloproteinase?2 (MMP?2) is important in the formation of liver fibrosis. In addition, HSCs are the primary cells secreting MMP?2 and extracellular matrix, therefore, there has been increasing interest in developing agents with high selectivity towards HSCs. However, no clinical drugs based on MMP?2, directed against HSCs, have been used to prevent fibrosis. Following consideration of the abundant vitamin A (VitA) receptors expressed on the cellular membrane of HSCs, the present study constructed VitA?coupled liposomes (VitA?lips) using dicyclohexylcarbodiimide?1, 3?diaminopentane condensation, rotatory film processing and ultrasonic oscillation. The results revealed that the liposomes exhibited low cytotoxicity and a suitable binding ability to MMP?2 small interference (si)RNA. Furthermore, the liposomes effectively delivered MMP?2 siRNA to the HSC?T6 cells. When HSCs were treated with the liposomes carrying MMP?2 siRNA (VitA?lip?MMP?2 siRNA), the mRNA expression and activity of MMP?2, and the protein expression levels of ??smooth muscle actin and type I collagen were significantly reduced. These results suggested that inhibition of the expression of MMP?2 in HSC?T6 cells may contribute to preventing hepatic fibrosis, and provided experimental support to the development of specific drugs against MMP?2 to prevent fibrogenesis in chronic liver disease. Twit Text FOAVip Inhibition of liver fibrosis using vitamin A-coupled liposomes to deliver matrix metalloproteinase-2 siRNA in vitro ????Mol Med Rep http://www.kidney.de/pget.php?&tw=1&pmid=26017616 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26017616 #FOAvip English Wikipedia <ref>{{Cite pmid|26017616 }}</ref> Inhibition of liver fibrosis using vitamin A-coupled liposomes to deliver matrix metalloproteinase-2 siRNA in vitro #MMPMID26017616 Li Y ; Liu F ; Ding F ; Chen P ; Tang M Mol Med Rep 2015[Sep]; 12 (3 ): 3453-3461 PMID26017616 show ga Hepatic fibrosis is a common form of wound healing in response to chronic liver injuries and can lead to more serious complications, including mortality. It is well?established that hepatic stellate cells (HSCs) are central mediators of hepatic fibrosis, and matrix metalloproteinase?2 (MMP?2) is important in the formation of liver fibrosis. In addition, HSCs are the primary cells secreting MMP?2 and extracellular matrix, therefore, there has been increasing interest in developing agents with high selectivity towards HSCs. However, no clinical drugs based on MMP?2, directed against HSCs, have been used to prevent fibrosis. Following consideration of the abundant vitamin A (VitA) receptors expressed on the cellular membrane of HSCs, the present study constructed VitA?coupled liposomes (VitA?lips) using dicyclohexylcarbodiimide?1, 3?diaminopentane condensation, rotatory film processing and ultrasonic oscillation. The results revealed that the liposomes exhibited low cytotoxicity and a suitable binding ability to MMP?2 small interference (si)RNA. Furthermore, the liposomes effectively delivered MMP?2 siRNA to the HSC?T6 cells. When HSCs were treated with the liposomes carrying MMP?2 siRNA (VitA?lip?MMP?2 siRNA), the mRNA expression and activity of MMP?2, and the protein expression levels of ??smooth muscle actin and type I collagen were significantly reduced. These results suggested that inhibition of the expression of MMP?2 in HSC?T6 cells may contribute to preventing hepatic fibrosis, and provided experimental support to the development of specific drugs against MMP?2 to prevent fibrogenesis in chronic liver disease. |Animals [MESH] |Apoptosis [MESH] |Cell Line [MESH] |Gene Knockdown Techniques [MESH] |Liposomes [MESH] |Liver Cirrhosis/*drug therapy [MESH] |Matrix Metalloproteinase 2/*genetics/metabolism [MESH] |RNA Interference [MESH] |RNA, Small Interfering/administration & dosage/*genetics [MESH] |Rats [MESH] |Transfection [MESH]Inhibition of liver fibrosis using vitamin A-coupled liposomes to deli(epmc).pdf DeepDyve Pubget Overpricing