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10.1136/gutjnl-2014-307979 http://scihub22266oqcxt.onion/10.1136/gutjnl-2014-307979 C4524790!4524790!25652085     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25652085&cmd=llinks): Failed to open stream: HTTP request failed! 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Kinase analysis in alcoholic hepatitis identifies p90RSK as a potential mediator of liver fibrogenesis |pdf=|usr=}}{{25652085}} gab.com Text Kinase analysis in alcoholic hepatitis identifies p90RSK as a potential mediator of liver fibrogenesis JO: Gut http://www.kidney.de/pget.php?&tw=1&pmid=25652085 #FOAvip Objective: Alcoholic hepatitis (AH) is often associated with advanced fibrosis, which negatively impacts survival. We aimed at identifying kinases deregulated in livers from patients with AH and advanced fibrosis in order to discover novel molecular targets. Design: Extensive phosphoprotein analysis by reverse phase protein microarrays was performed in AH (n=12) and normal human livers (n=7). Ribosomal S6 kinase (p90RSK) hepatic expression was assessed by qPCR, Western blot and immunohistochemistry. Kaempferol was used as a selective pharmacological inhibitor of the p90RSK pathway to assess the regulation of experimentally-induced liver fibrosis and injury, using in vivo and in vitro approaches. Results: Proteomic analysis identified p90RSK as one of the most deregulated kinases in AH. Hepatic p90RSK gene and protein expression was also upregulated in livers with chronic liver disease. Immunohistochemistry studies showed increased p90RSK staining in areas of active fibrogenesis in cirrhotic livers. Therapeutic administration of kaempferol to carbon tetrachloride-treated mice resulted in decreased hepatic collagen deposition, and expression of profibrogenic and proinflammatory genes, compared to vehicle administration. In addition, kaempferol reduced the extent of hepatocellular injury and degree of apoptosis. In primary hepatic stellate cells, kaempferol and small interfering RNA decreased activation of p90RSK, which in turn regulated key profibrogenic actions. In primary hepatocytes, kaempferol attenuated proapoptotic signalling. Conclusions: p90RSK is upregulated in patients with chronic liver disease and mediates liver fibrogenesis in vivo and in vitro. These results suggest that the p90RSK pathway could be a new therapeutic approach for liver diseases characterised by advanced fibrosis. Twit Text FOAVip Kinase analysis in alcoholic hepatitis identifies p90RSK as a potential mediator of liver fibrogenesis ????Gut http://www.kidney.de/pget.php?&tw=1&pmid=25652085 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25652085 #FOAvip English Wikipedia <ref>{{Cite pmid|25652085}}</ref> Kinase analysis in alcoholic hepatitis identifies p90RSK as a potential mediator of liver fibrogenesis #MMPMID25652085 Morales-Ibanez O; Affò S; Rodrigo-Torres D; Blaya D; Millán C; Coll M; Perea L; Odena G; Knorpp T; Templin MF; Moreno M; Altamirano J; Miquel R; Arroyo V; Ginès P; Caballería J; Sancho-Bru P; Bataller R Gut 2016[May]; 65 (5): 840-51 PMID25652085show ga Objective: Alcoholic hepatitis (AH) is often associated with advanced fibrosis, which negatively impacts survival. We aimed at identifying kinases deregulated in livers from patients with AH and advanced fibrosis in order to discover novel molecular targets. Design: Extensive phosphoprotein analysis by reverse phase protein microarrays was performed in AH (n=12) and normal human livers (n=7). Ribosomal S6 kinase (p90RSK) hepatic expression was assessed by qPCR, Western blot and immunohistochemistry. Kaempferol was used as a selective pharmacological inhibitor of the p90RSK pathway to assess the regulation of experimentally-induced liver fibrosis and injury, using in vivo and in vitro approaches. Results: Proteomic analysis identified p90RSK as one of the most deregulated kinases in AH. Hepatic p90RSK gene and protein expression was also upregulated in livers with chronic liver disease. Immunohistochemistry studies showed increased p90RSK staining in areas of active fibrogenesis in cirrhotic livers. Therapeutic administration of kaempferol to carbon tetrachloride-treated mice resulted in decreased hepatic collagen deposition, and expression of profibrogenic and proinflammatory genes, compared to vehicle administration. In addition, kaempferol reduced the extent of hepatocellular injury and degree of apoptosis. In primary hepatic stellate cells, kaempferol and small interfering RNA decreased activation of p90RSK, which in turn regulated key profibrogenic actions. In primary hepatocytes, kaempferol attenuated proapoptotic signalling. Conclusions: p90RSK is upregulated in patients with chronic liver disease and mediates liver fibrogenesis in vivo and in vitro. These results suggest that the p90RSK pathway could be a new therapeutic approach for liver diseases characterised by advanced fibrosis. äKinase analysis in alcoholic hepatitis identifies p90RSK as a potentia(epmc).pdf DeepDyve Pubget Overpricing