Tsai PS; Mateo C; Field JJ; Schaffer CB; Anderson ME; Kleinfeld D
Opt Express 2015[Jun]; 23 (11): 13833-47 PMID26072755show ga
We present a two-photon microscope that images the full extent of murine cortex with an objective-limited spatial resolution across an 8 mm by 10 mm field. The lateral resolution is approximately 1 µm and the maximum scan speed is 5 mm/ms. The scan pathway employs large diameter compound lenses to minimize aberrations and performs near theoretical limits. We demonstrate the special utility of the microscope by recording resting-state vasomotion across both hemispheres of the murine brain through a transcranial window and by imaging histological sections without the need to stitch.