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10.1089/ten.tec.2014.0396 http://scihub22266oqcxt.onion/10.1089/ten.tec.2014.0396 C4523041!4523041!25654448     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Tissue+Eng+Part+C+Methods 2015 ; 21 (8): 832-40 Nephropedia Template TP {{tp|p=25654448|t=2015. Multiple-Step Injection Molding for Fibrin-Based Tissue-Engineered Heart Valves |pdf=|usr=}}{{25654448}} gab.com Text Multiple-Step Injection Molding for Fibrin-Based Tissue-Engineered Heart Valves JO: Tissue Eng Part C Methods http://www.kidney.de/pget.php?&tw=1&pmid=25654448 #FOAvip Heart valves are elaborate and highly heterogeneous structures of the circulatory system. Despite the well accepted relationship between the structural and mechanical anisotropy and the optimal function of the valves, most approaches to create tissue-engineered heart valves (TEHVs) do not try to mimic this complexity and rely on one homogenous combination of cells and materials for the whole construct. The aim of this study was to establish an easy and versatile method to introduce spatial diversity into a heart valve fibrin scaffold. We developed a multiple-step injection molding process that enables the fabrication of TEHVs with heterogeneous composition (cell/scaffold material) of wall and leaflets without the need of gluing or suturing components together, with the leaflets firmly connected to the wall. The integrity of the valves and their functionality was proved by either opening/closing cycles in a bioreactor (proof of principle without cells) or with continuous stimulation over 2 weeks. We demonstrated the potential of the method by the two-step molding of the wall and the leaflets containing different cell lines. Immunohistology after stimulation confirmed tissue formation and demonstrated the localization of the different cell types. Furthermore, we showed the proof of principle fabrication of valves using different materials for wall (fibrin) and leaflets (hybrid gel of fibrin/elastin-like recombinamer) and with layered leaflets. The method is easy to implement, does not require special facilities, and can be reproduced in any tissue-engineering lab. While it has been demonstrated here with fibrin, it can easily be extended to other hydrogels. Twit Text FOAVip Multiple-Step Injection Molding for Fibrin-Based Tissue-Engineered Heart Valves ????Tissue Eng Part C Methods http://www.kidney.de/pget.php?&tw=1&pmid=25654448 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25654448 #FOAvip English Wikipedia <ref>{{Cite pmid|25654448}}</ref> Multiple-Step Injection Molding for Fibrin-Based Tissue-Engineered Heart Valves #MMPMID25654448 Weber M; Gonzalez de Torre I; Moreira R; Frese J; Oedekoven C; Alonso M; Rodriguez Cabello CJ; Jockenhoevel S; Mela P Tissue Eng Part C Methods 2015[Aug]; 21 (8): 832-40 PMID25654448show ga Heart valves are elaborate and highly heterogeneous structures of the circulatory system. Despite the well accepted relationship between the structural and mechanical anisotropy and the optimal function of the valves, most approaches to create tissue-engineered heart valves (TEHVs) do not try to mimic this complexity and rely on one homogenous combination of cells and materials for the whole construct. The aim of this study was to establish an easy and versatile method to introduce spatial diversity into a heart valve fibrin scaffold. We developed a multiple-step injection molding process that enables the fabrication of TEHVs with heterogeneous composition (cell/scaffold material) of wall and leaflets without the need of gluing or suturing components together, with the leaflets firmly connected to the wall. The integrity of the valves and their functionality was proved by either opening/closing cycles in a bioreactor (proof of principle without cells) or with continuous stimulation over 2 weeks. We demonstrated the potential of the method by the two-step molding of the wall and the leaflets containing different cell lines. Immunohistology after stimulation confirmed tissue formation and demonstrated the localization of the different cell types. Furthermore, we showed the proof of principle fabrication of valves using different materials for wall (fibrin) and leaflets (hybrid gel of fibrin/elastin-like recombinamer) and with layered leaflets. The method is easy to implement, does not require special facilities, and can be reproduced in any tissue-engineering lab. While it has been demonstrated here with fibrin, it can easily be extended to other hydrogels. äMultiple-Step Injection Molding for Fibrin-Based Tissue-Engineered Hea(epmc).pdf DeepDyve Pubget Overpricing