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10.1021/jacs.5b02109 http://scihub22266oqcxt.onion/10.1021/jacs.5b02109 C4519080!4519080 !25825995     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25825995 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Am+Chem+Soc 2015 ; 137 (15 ): 5225-30 Nephropedia Template TP {{tp|p=25825995 |t=2015. Viewing Human DNA Polymerase ? Faithfully and Unfaithfully Bypass an Oxidative Lesion by Time-Dependent Crystallography |pdf=|usr=}}{{25825995 }} gab.com Text Viewing Human DNA Polymerase Faithfully and Unfaithfully Bypass an Oxidative Lesion by Time-Dependent Crystallography JO: J Am Chem Soc http://www.kidney.de/pget.php?&tw=1&pmid=25825995 #FOAvip One common oxidative DNA lesion, 8-oxo-7,8-dihydro-2'-deoxyguanine (8-oxoG), is highly mutagenic in vivo due to its anti-conformation forming a Watson-Crick base pair with correct deoxycytidine 5'-triphosphate (dCTP) and its syn-conformation forming a Hoogsteen base pair with incorrect deoxyadenosine 5'-triphosphate (dATP). Here, we utilized time-resolved X-ray crystallography to follow 8-oxoG bypass by human DNA polymerase ? (hPol?). In the 12 solved structures, both Watson-Crick (anti-8-oxoG:anti-dCTP) and Hoogsteen (syn-8-oxoG:anti-dATP) base pairing were clearly visible and were maintained throughout the chemical reaction. Additionally, a third Mg(2+) appeared during the process of phosphodiester bond formation and was located between the reacting ?- and ?-phosphates of the dNTP, suggesting its role in stabilizing reaction intermediates. After phosphodiester bond formation, hPol? reopened its conformation, pyrophosphate was released, and the newly incorporated primer 3'-terminal nucleotide stacked, rather than base paired, with 8-oxoG. These structures provide the first real-time pictures, to our knowledge, of how a polymerase correctly and incorrectly bypasses a DNA lesion. Twit Text FOAVip Viewing Human DNA Polymerase Faithfully and Unfaithfully Bypass an Oxidative Lesion by Time-Dependent Crystallography ????J Am Chem Soc http://www.kidney.de/pget.php?&tw=1&pmid=25825995 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25825995 #FOAvip English Wikipedia <ref>{{Cite pmid|25825995 }}</ref> Viewing Human DNA Polymerase ? Faithfully and Unfaithfully Bypass an Oxidative Lesion by Time-Dependent Crystallography #MMPMID25825995 Vyas R ; Reed AJ ; Tokarsky EJ ; Suo Z J Am Chem Soc 2015[Apr]; 137 (15 ): 5225-30 PMID25825995 show ga One common oxidative DNA lesion, 8-oxo-7,8-dihydro-2'-deoxyguanine (8-oxoG), is highly mutagenic in vivo due to its anti-conformation forming a Watson-Crick base pair with correct deoxycytidine 5'-triphosphate (dCTP) and its syn-conformation forming a Hoogsteen base pair with incorrect deoxyadenosine 5'-triphosphate (dATP). Here, we utilized time-resolved X-ray crystallography to follow 8-oxoG bypass by human DNA polymerase ? (hPol?). In the 12 solved structures, both Watson-Crick (anti-8-oxoG:anti-dCTP) and Hoogsteen (syn-8-oxoG:anti-dATP) base pairing were clearly visible and were maintained throughout the chemical reaction. Additionally, a third Mg(2+) appeared during the process of phosphodiester bond formation and was located between the reacting ?- and ?-phosphates of the dNTP, suggesting its role in stabilizing reaction intermediates. After phosphodiester bond formation, hPol? reopened its conformation, pyrophosphate was released, and the newly incorporated primer 3'-terminal nucleotide stacked, rather than base paired, with 8-oxoG. These structures provide the first real-time pictures, to our knowledge, of how a polymerase correctly and incorrectly bypasses a DNA lesion. |8-Hydroxy-2'-Deoxyguanosine/analogs & derivatives [MESH] |Biocatalysis [MESH] |Catalytic Domain [MESH] |Crystallography, X-Ray [MESH] |DNA Damage [MESH] |DNA Polymerase beta/*chemistry/*metabolism [MESH] |Diphosphates/chemistry/metabolism [MESH] |Guanine/analogs & derivatives/chemistry/metabolism [MESH] |Humans [MESH] |Metals/chemistry/metabolism [MESH] |Models, Molecular [MESH] |Oxidation-Reduction [MESH] |Protein Conformation [MESH]Viewing Human DNA Polymerase ? Faithfully and Unfaithfully Bypass an O(epmc).pdf DeepDyve Pubget Overpricing