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10.1371/journal.pone.0133757 http://scihub22266oqcxt.onion/10.1371/journal.pone.0133757 C4517886!4517886 !26218078     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26218078 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       PLoS+One 2015 ; 10 (7 ): e0133757 Nephropedia Template TP {{tp|p=26218078 |t=2015. The Energy of COPI for Budding Membranes |pdf=|usr=}}{{26218078 }} gab.com Text The Energy of COPI for Budding Membranes JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=26218078 #FOAvip As a major actor of cellular trafficking, COPI coat proteins assemble on membranes and locally bend them to bud 60 nm-size coated particles. Budding requires the energy of the coat assembly to overcome the one necessary to deform the membrane which primarily depends on the bending modulus and surface tension, ?. Using a COPI-induced oil nanodroplet formation approach, we modulated the budding of nanodroplets using various amounts and types of surfactant. We found a Heaviside-like dependence between the budding efficiency and ?: budding was only dependent on ? and occurred beneath 1.3 mN/m. With the sole contribution of ? to the membrane deformation energy, we assessed that COPI supplies ~1500 kBT for budding particles from membranes, which is consistent with common membrane deformation energies. Our results highlight how a simple remodeling of the composition of membranes could mechanically modulate budding in cells. Twit Text FOAVip The Energy of COPI for Budding Membranes ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=26218078 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26218078 #FOAvip English Wikipedia <ref>{{Cite pmid|26218078 }}</ref> The Energy of COPI for Budding Membranes #MMPMID26218078 Thiam AR ; Pincet F PLoS One 2015[]; 10 (7 ): e0133757 PMID26218078 show ga As a major actor of cellular trafficking, COPI coat proteins assemble on membranes and locally bend them to bud 60 nm-size coated particles. Budding requires the energy of the coat assembly to overcome the one necessary to deform the membrane which primarily depends on the bending modulus and surface tension, ?. Using a COPI-induced oil nanodroplet formation approach, we modulated the budding of nanodroplets using various amounts and types of surfactant. We found a Heaviside-like dependence between the budding efficiency and ?: budding was only dependent on ? and occurred beneath 1.3 mN/m. With the sole contribution of ? to the membrane deformation energy, we assessed that COPI supplies ~1500 kBT for budding particles from membranes, which is consistent with common membrane deformation energies. Our results highlight how a simple remodeling of the composition of membranes could mechanically modulate budding in cells. |*Energy Metabolism [MESH] |Biological Transport [MESH] |COP-Coated Vesicles/*physiology [MESH] |Cell Membrane/*physiology [MESH] |Coat Protein Complex I/*metabolism [MESH] |Humans [MESH]The Energy of COPI for Budding Membranes (epmc).pdf DeepDyve Pubget Overpricing