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10.1371/journal.pone.0134092 http://scihub22266oqcxt.onion/10.1371/journal.pone.0134092 C4517793!4517793 !26217927     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26217927 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       PLoS+One 2015 ; 10 (7 ): e0134092 Nephropedia Template TP {{tp|p=26217927 |t=2015. Partially Evoked Epithelial-Mesenchymal Transition (EMT) Is Associated with Increased TGF? Signaling within Lesional Scleroderma Skin |pdf=|usr=}}{{26217927 }} gab.com Text Partially Evoked Epithelial-Mesenchymal Transition (EMT) Is Associated with Increased TGF Signaling within Lesional Scleroderma Skin JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=26217927 #FOAvip The origin of myofibroblasts in fibrotic conditions remains unknown and in systemic sclerosis (SSc) it has been proposed that activation of local fibroblasts, trans-differentiation of perivascular or vascular cells, recruitment of fibrocyte progenitors, or epithelial to mesenchymal transition (EMT) could be contributing. Data from our laboratory indicate that the epidermis in scleroderma is activated with the keratinocytes exhibiting a phenotype normally associated with tissue repair, including phosphorylation profiles indicative of TGF? signaling. Since TGF? is a known inducer of EMT, we investigated if there is evidence of this process in the SSc epidermis. In order to validate antibodies and primers, EMT was modeled in HaCaT cells cultured in the presence of TGF?1. Skin sections were stained with phosho-SMAD2/3, as well as with epithelial and mesenchymal markers. Moreover, mRNA levels of transcription factors associated with EMT were studied in epidermal blister sheets. We observed critical changes in the scleroderma epidermis; showing significantly increased nuclear translocation of phosphorylated Smad2/3, consistent with active TGF? signaling in SSc keratinocytes. While profound EMT could be induced in keratinocytes in vitro with the appearance of SNAI1/2 and FSP-1, and an accompanying loss of E-cadherin, in the scleroderma skin active TGF? signaling was accompanied by only partial EMT-like changes characterised by induction of SNAI1 alone and with no loss of E-cadherin. Together, our findings support a model of altered differentiation and TGF? dependent activation of scleroderma epithelial cells leading to a partially evoked EMT like process in the fibrotic skin. Twit Text FOAVip Partially Evoked Epithelial-Mesenchymal Transition (EMT) Is Associated with Increased TGF Signaling within Lesional Scleroderma Skin ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=26217927 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26217927 #FOAvip English Wikipedia <ref>{{Cite pmid|26217927 }}</ref> Partially Evoked Epithelial-Mesenchymal Transition (EMT) Is Associated with Increased TGF? Signaling within Lesional Scleroderma Skin #MMPMID26217927 Nikitorowicz-Buniak J ; Denton CP ; Abraham D ; Stratton R PLoS One 2015[]; 10 (7 ): e0134092 PMID26217927 show ga The origin of myofibroblasts in fibrotic conditions remains unknown and in systemic sclerosis (SSc) it has been proposed that activation of local fibroblasts, trans-differentiation of perivascular or vascular cells, recruitment of fibrocyte progenitors, or epithelial to mesenchymal transition (EMT) could be contributing. Data from our laboratory indicate that the epidermis in scleroderma is activated with the keratinocytes exhibiting a phenotype normally associated with tissue repair, including phosphorylation profiles indicative of TGF? signaling. Since TGF? is a known inducer of EMT, we investigated if there is evidence of this process in the SSc epidermis. In order to validate antibodies and primers, EMT was modeled in HaCaT cells cultured in the presence of TGF?1. Skin sections were stained with phosho-SMAD2/3, as well as with epithelial and mesenchymal markers. Moreover, mRNA levels of transcription factors associated with EMT were studied in epidermal blister sheets. We observed critical changes in the scleroderma epidermis; showing significantly increased nuclear translocation of phosphorylated Smad2/3, consistent with active TGF? signaling in SSc keratinocytes. While profound EMT could be induced in keratinocytes in vitro with the appearance of SNAI1/2 and FSP-1, and an accompanying loss of E-cadherin, in the scleroderma skin active TGF? signaling was accompanied by only partial EMT-like changes characterised by induction of SNAI1 alone and with no loss of E-cadherin. Together, our findings support a model of altered differentiation and TGF? dependent activation of scleroderma epithelial cells leading to a partially evoked EMT like process in the fibrotic skin. |*Epithelial-Mesenchymal Transition [MESH] |Case-Control Studies [MESH] |Cell Proliferation [MESH] |Cells, Cultured [MESH] |Fluorescent Antibody Technique [MESH] |Humans [MESH] |Immunoenzyme Techniques [MESH] |Keratinocytes/cytology/*metabolism [MESH] |Phosphorylation [MESH] |RNA, Messenger/genetics [MESH] |Real-Time Polymerase Chain Reaction [MESH] |Reverse Transcriptase Polymerase Chain Reaction [MESH] |Scleroderma, Systemic/genetics/metabolism/*pathology [MESH] |Skin Diseases/genetics/metabolism/*pathology [MESH]Partially Evoked Epithelial-Mesenchymal Transition (EMT) Is Associated(epmc).pdf DeepDyve Pubget Overpricing