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10.15252/embj.201590931 http://scihub22266oqcxt.onion/10.15252/embj.201590931 C4516432!4516432!25979828     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       EMBO+J 2015 ; 34 (13): 1801-15 Nephropedia Template TP {{tp|p=25979828|t=2015. TUT7 controls the fate of precursor microRNAs by using three different uridylation mechanisms |pdf=|usr=}}{{25979828}} gab.com Text TUT7 controls the fate of precursor microRNAs by using three different uridylation mechanisms JO: EMBO J http://www.kidney.de/pget.php?&tw=1&pmid=25979828 #FOAvip Terminal uridylyl transferases (TUTs) function as integral regulators of microRNA (miRNA) biogenesis. Using biochemistry, single-molecule, and deep sequencing techniques, we here investigate the mechanism by which human TUT7 (also known as ZCCHC6) recognizes and uridylates precursor miRNAs (pre-miRNAs) in the absence of Lin28. We find that the overhang of a pre-miRNA is the key structural element that is recognized by TUT7 and its paralogues, TUT4 (ZCCHC11) and TUT2 (GLD2/PAPD4). For group II pre-miRNAs, which have a 1-nt 3? overhang, TUT7 restores the canonical end structure (2-nt 3? overhang) through mono-uridylation, thereby promoting miRNA biogenesis. For pre-miRNAs where the 3? end is further recessed into the stem (as in 3? trimmed pre-miRNAs), TUT7 generates an oligo-U tail that leads to degradation. In contrast to Lin28-stimulated oligo-uridylation, which is processive, a distributive mode is employed by TUT7 for both mono- and oligo-uridylation in the absence of Lin28. The overhang length dictates the frequency (but not duration) of the TUT7-RNA interaction, thus explaining how TUT7 differentiates pre-miRNA species with different overhangs. Our study reveals dual roles and mechanisms of uridylation in repair and removal of defective pre-miRNAs. Twit Text FOAVip TUT7 controls the fate of precursor microRNAs by using three different uridylation mechanisms ????EMBO J http://www.kidney.de/pget.php?&tw=1&pmid=25979828 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25979828 #FOAvip English Wikipedia <ref>{{Cite pmid|25979828}}</ref> TUT7 controls the fate of precursor microRNAs by using three different uridylation mechanisms #MMPMID25979828 Kim B; Ha M; Loeff L; Chang H; Simanshu DK; Li S; Fareh M; Patel DJ; Joo C; Kim VN EMBO J 2015[Jul]; 34 (13): 1801-15 PMID25979828show ga Terminal uridylyl transferases (TUTs) function as integral regulators of microRNA (miRNA) biogenesis. Using biochemistry, single-molecule, and deep sequencing techniques, we here investigate the mechanism by which human TUT7 (also known as ZCCHC6) recognizes and uridylates precursor miRNAs (pre-miRNAs) in the absence of Lin28. We find that the overhang of a pre-miRNA is the key structural element that is recognized by TUT7 and its paralogues, TUT4 (ZCCHC11) and TUT2 (GLD2/PAPD4). For group II pre-miRNAs, which have a 1-nt 3? overhang, TUT7 restores the canonical end structure (2-nt 3? overhang) through mono-uridylation, thereby promoting miRNA biogenesis. For pre-miRNAs where the 3? end is further recessed into the stem (as in 3? trimmed pre-miRNAs), TUT7 generates an oligo-U tail that leads to degradation. In contrast to Lin28-stimulated oligo-uridylation, which is processive, a distributive mode is employed by TUT7 for both mono- and oligo-uridylation in the absence of Lin28. The overhang length dictates the frequency (but not duration) of the TUT7-RNA interaction, thus explaining how TUT7 differentiates pre-miRNA species with different overhangs. Our study reveals dual roles and mechanisms of uridylation in repair and removal of defective pre-miRNAs. äTUT7 controls the fate of precursor microRNAs by using three different(epmc).pdf DeepDyve Pubget Overpricing