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2009 ; 13
(11-12
): 4484-91
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gab.com Text
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Renal, vascular and cardiac fibrosis in rats exposed to passive smoking and
industrial dust fibre amosite
#MMPMID19292733
Boor P
; Casper S
; Celec P
; Hurbánková M
; Beno M
; Heidland A
; Amann K
; Sebeková K
J Cell Mol Med
2009[Nov]; 13
(11-12
): 4484-91
PMID19292733
show ga
Passive smoking is an independent risk factor for cardiovascular diseases.
Industrial fibrous dust, e.g. the asbestos group member, amosite, causes lung
cancer and fibrosis. No data are available on renal involvement after
inhalational exposure to these environmental pollutants or of their combination,
or on cardiovascular and renal toxicity after exposure to amosite. Male Wistar
rats were randomized into four groups (n= 6): control and amosite group received
initially two intratracheal instillations of saline and amosite solution,
respectively. Smoking group was subjected to standardized daily exposure to
tobacco smoke for 2 hrs in a concentration resembling human passive smoking.
Combined group was exposed to both amosite and cigarette smoke. All rats were
killed after 6 months. Rats exposed to either amosite or passive smoking
developed significant glomerulosclerosis and tubulointerstitial fibrosis.
Combination of both exposures had additive effects. Histomorphological changes
preceded the clinical manifestation of kidney damage. In both groups with single
exposures, marked perivascular and interstitial cardiac fibrosis was detected.
The additive effect in the heart was less pronounced than in the kidney, apparent
particularly in changes of vascular structure. Advanced oxidation protein
products, the plasma marker of the myeloperoxidase reaction in activated
monocytes/macrophages, were increased in all exposed groups, whereas the
inflammatory cytokines did not differ between the groups. In rats, passive
smoking or amosite instillation leads to renal, vascular and cardiac fibrosis
potentially mediated via increased myeloperoxidase reaction. Combination of both
pollutants shows additive effects. Our data should be confirmed in subjects
exposed to these environmental pollutants, in particular if combined.