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10.1080/10739680601139179

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suck abstract from ncbi


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pmid17454670
      Microcirculation 2007 ; 14 (3 ): 169-80
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  • Noninvasive measure of microvascular nitric oxide function in humans using very low-frequency cutaneous laser Doppler flow spectra #MMPMID17454670
  • Stewart JM ; Taneja I ; Goligorsky MS ; Medow MS
  • Microcirculation 2007[Apr]; 14 (3 ): 169-80 PMID17454670 show ga
  • OBJECTIVE: While higher frequency oscillations (0.021-0.6 Hz) in cutaneous blood flow measured by laser Doppler flowmetry (LDF) relate to oscillations in blood pressure and sympathetic nerve activity, very low-frequency oscillations (VLF, 0.0095-0.021 Hz) do not. The authors investigated whether VLF LDF power is nitric oxide (NO) specific. METHODS: LDF combined with intradermal microdialysis was used in the calves of 22 healthy volunteers aged 19-27 years. LDF power spectral analysis was performed by windowed fast Fourier transform. The authors tested whether the NO synthesis inhibitor nitro-l-arginine (NLA) produced selective decreases in VLF power before and after stimulation with acetylcholine. RESULTS: NLA alone did not alter total power but selectively reduced VLF power by approximately 50%. LDF and spectral power increased markedly across all spectra with acetylcholine. This increase was blunted by NLA, which selectively reduced VLF power by approximately 50%. CONCLUSIONS: The data suggest that VLF oscillations in the laser Doppler signal are NO dependent, increase with cholinergic stimulation, and have potential as a noninvasive marker for NO-dependent microvascular reactivity.
  • |*Laser-Doppler Flowmetry [MESH]
  • |Acetylcholine/*administration & dosage [MESH]
  • |Adult [MESH]
  • |Blood Pressure/drug effects [MESH]
  • |Enzyme Inhibitors/*administration & dosage [MESH]
  • |Female [MESH]
  • |Humans [MESH]
  • |Male [MESH]
  • |Nitric Oxide/*blood [MESH]
  • |Nitroarginine/*administration & dosage [MESH]
  • |Skin/blood supply/metabolism [MESH]
  • |Sympathetic Nervous System/physiology [MESH]


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