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Noninvasive Measure of Microvascular Nitric Oxide Function in Humans Using Very Low-Frequency Cutaneous Laser Doppler Flow Spectra #MMPMID17454670
STEWART JM; TANEJA I; GOLIGORSKY MS; MEDOW MS
Microcirculation 2007[Apr]; 14 (3): 169-80 PMID17454670show ga
Objective: While higher frequency oscillations (0.021?0.6 Hz) in cutaneous blood flow measured by laser Doppler flowmetry (LDF) relate to oscillations in blood pressure and sympathetic nerve activity, very low-frequency oscillations (VLF, 0.0095?0.021 Hz) do not. The authors investigated whether VLF LDF power is nitric oxide (NO) specific. Methods: LDF combined with intradermal microdialysis was used in the calves of 22 healthy volunteers aged 19?27 years. LDF power spectral analysis was performed by windowed fast Fourier transform. The authors tested whether the NO synthesis inhibitor nitro-l-arginine (NLA) produced selective decreases in VLF power before and after stimulation with acetylcholine. Results: NLA alone did not alter total power but selectively reduced VLF power by approximately 50%. LDF and spectral power increased markedly across all spectra with acetylcholine. This increase was blunted by NLA, which selectively reduced VLF power by approximately 50%. Conclusions: The data suggest that VLF oscillations in the laser Doppler signal are NO dependent, increase with cholinergic stimulation, and have potential as a noninvasive marker for NO-dependent microvascular reactivity.