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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Microcirculation
2007 ; 14
(3
): 169-80
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Noninvasive measure of microvascular nitric oxide function in humans using very
low-frequency cutaneous laser Doppler flow spectra
#MMPMID17454670
Stewart JM
; Taneja I
; Goligorsky MS
; Medow MS
Microcirculation
2007[Apr]; 14
(3
): 169-80
PMID17454670
show ga
OBJECTIVE: While higher frequency oscillations (0.021-0.6 Hz) in cutaneous blood
flow measured by laser Doppler flowmetry (LDF) relate to oscillations in blood
pressure and sympathetic nerve activity, very low-frequency oscillations (VLF,
0.0095-0.021 Hz) do not. The authors investigated whether VLF LDF power is nitric
oxide (NO) specific. METHODS: LDF combined with intradermal microdialysis was
used in the calves of 22 healthy volunteers aged 19-27 years. LDF power spectral
analysis was performed by windowed fast Fourier transform. The authors tested
whether the NO synthesis inhibitor nitro-l-arginine (NLA) produced selective
decreases in VLF power before and after stimulation with acetylcholine. RESULTS:
NLA alone did not alter total power but selectively reduced VLF power by
approximately 50%. LDF and spectral power increased markedly across all spectra
with acetylcholine. This increase was blunted by NLA, which selectively reduced
VLF power by approximately 50%. CONCLUSIONS: The data suggest that VLF
oscillations in the laser Doppler signal are NO dependent, increase with
cholinergic stimulation, and have potential as a noninvasive marker for
NO-dependent microvascular reactivity.