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10.1126/scitranslmed.aaa3519 http://scihub22266oqcxt.onion/10.1126/scitranslmed.aaa3519 C4511399!4511399!26019220     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Transl+Med 2015 ; 7 (289): 289ra84 Nephropedia Template TP {{tp|p=26019220|t=2015. Programmable probiotics for detection of cancer in urine |pdf=|usr=}}{{26019220}} gab.com Text Programmable probiotics for detection of cancer in urine JO: Sci Transl Med http://www.kidney.de/pget.php?&tw=1&pmid=26019220 #FOAvip Rapid advances in the forward engineering of genetic circuitry in living cells has positioned synthetic biology as a potential means to solve numerous biomedical problems, including disease diagnosis and therapy. One challenge in exploiting synthetic biology for translational applications is to engineer microbes that are well tolerated by patients and seamlessly integrate with existing clinical methods. We use the safe and widely used probiotic Escherichia coli Nissle 1917 to develop an orally administered diagnostic that can noninvasively indicate the presence of liver metastasis by producing easily detectable signals in urine. Our microbial diagnostic generated a high-contrast urine signal through selective expansion in liver metastases (106-fold enrichment) and high expression of a lacZ reporter maintained by engineering a stable plasmid system. The lacZ reporter cleaves a substrate to produce a small molecule that can be detected in urine. E. coli Nissle 1917 robustly colonized tumor tissue in rodent models of liver metastasis after oral delivery but did not colonize healthy organs or fibrotic liver tissue. We saw no deleterious health effects on the mice for more than 12 months after oral delivery. Our results demonstrate that probiotics can be programmed to safely and selectively deliver synthetic gene circuits to diseased tissue microenvironments in vivo. Twit Text FOAVip Programmable probiotics for detection of cancer in urine ????Sci Transl Med http://www.kidney.de/pget.php?&tw=1&pmid=26019220 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26019220 #FOAvip English Wikipedia <ref>{{Cite pmid|26019220}}</ref> Programmable probiotics for detection of cancer in urine #MMPMID26019220 Danino T; Prindle A; Kwong GA; Skalak M; Li H; Allen K; Hasty J; Bhatia SN Sci Transl Med 2015[May]; 7 (289): 289ra84 PMID26019220show ga Rapid advances in the forward engineering of genetic circuitry in living cells has positioned synthetic biology as a potential means to solve numerous biomedical problems, including disease diagnosis and therapy. One challenge in exploiting synthetic biology for translational applications is to engineer microbes that are well tolerated by patients and seamlessly integrate with existing clinical methods. We use the safe and widely used probiotic Escherichia coli Nissle 1917 to develop an orally administered diagnostic that can noninvasively indicate the presence of liver metastasis by producing easily detectable signals in urine. Our microbial diagnostic generated a high-contrast urine signal through selective expansion in liver metastases (106-fold enrichment) and high expression of a lacZ reporter maintained by engineering a stable plasmid system. The lacZ reporter cleaves a substrate to produce a small molecule that can be detected in urine. E. coli Nissle 1917 robustly colonized tumor tissue in rodent models of liver metastasis after oral delivery but did not colonize healthy organs or fibrotic liver tissue. We saw no deleterious health effects on the mice for more than 12 months after oral delivery. Our results demonstrate that probiotics can be programmed to safely and selectively deliver synthetic gene circuits to diseased tissue microenvironments in vivo. äProgrammable probiotics for detection of cancer in urine (epmc).pdf DeepDyve Pubget Overpricing