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10.4137/GEI.S9125 http://scihub22266oqcxt.onion/10.4137/GEI.S9125 C4510604!4510604!26217105     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Genomics+Insights 2012 ; 5 (ä): 1-12 Nephropedia Template TP {{tp|p=26217105|t=2012. Transcriptional Changes of Blood Eosinophils After Methacholine Inhalation Challenge in Asthmatics |pdf=|usr=}}{{26217105}} gab.com Text Transcriptional Changes of Blood Eosinophils After Methacholine Inhalation Challenge in Asthmatics JO: Genomics Insights http://www.kidney.de/pget.php?&tw=1&pmid=26217105 #FOAvip Background: Methacholine challenge is commonly used within the asthma diagnostic algorithm. Methacholine challenge has recently been shown to induce airway remodelling in asthma via bronchoconstriction, without additional airway inflammation. We evaluated the effect of methacholine-induced bronchoconstriction on the peripheral whole-blood transcriptome. Methods: Fourteen males with adult-onset, occupational asthma, 26?77 years of age, underwent methacholine inhalation challenges. The concentration of methacholine eliciting a ?20% fall in FEV1 (PC20) was determined. Blood was collected immediately prior to and two hours after challenge. Complete blood counts and leukocyte differentials were obtained. Transcriptome analysis was performed using Affymetrix GeneChip® Human Gene 1.0 ST arrays. Data were analyzed using robust LIMMA and SAM. The cell-specific Significance Analysis of Microarrays (csSAM) algorithm was used to deconvolute the gene expression data according to cell type. Results: Microarray pathway analysis indicated that inflammatory processes were differentially affected. CsSAM identified 1,559 transcripts differentially expressed (all down-regulated) between pre- and post-methacholine in eosinophils at a false discovery cutoff of 10%. Notable changes included the GOLGA5 and METTL2B genes and the protein ubiquitination and CCR3 pathways. Conclusions: We demonstrated significant changes in the peripheral blood eosinophil-specific transcriptome of asthmatics two hours after methacholine challenge. CCR3 and protein ubiquitination pathways are both significantly down-regulated. Twit Text FOAVip Transcriptional Changes of Blood Eosinophils After Methacholine Inhalation Challenge in Asthmatics ????Genomics Insights http://www.kidney.de/pget.php?&tw=1&pmid=26217105 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26217105 #FOAvip English Wikipedia <ref>{{Cite pmid|26217105}}</ref> Transcriptional Changes of Blood Eosinophils After Methacholine Inhalation Challenge in Asthmatics #MMPMID26217105 Tebbutt SJ; He JQ; Singh A; Shannon CP; Ruan J; Carlsten C Genomics Insights 2012[]; 5 (ä): 1-12 PMID26217105show ga Background: Methacholine challenge is commonly used within the asthma diagnostic algorithm. Methacholine challenge has recently been shown to induce airway remodelling in asthma via bronchoconstriction, without additional airway inflammation. We evaluated the effect of methacholine-induced bronchoconstriction on the peripheral whole-blood transcriptome. Methods: Fourteen males with adult-onset, occupational asthma, 26?77 years of age, underwent methacholine inhalation challenges. The concentration of methacholine eliciting a ?20% fall in FEV1 (PC20) was determined. Blood was collected immediately prior to and two hours after challenge. Complete blood counts and leukocyte differentials were obtained. Transcriptome analysis was performed using Affymetrix GeneChip® Human Gene 1.0 ST arrays. Data were analyzed using robust LIMMA and SAM. The cell-specific Significance Analysis of Microarrays (csSAM) algorithm was used to deconvolute the gene expression data according to cell type. Results: Microarray pathway analysis indicated that inflammatory processes were differentially affected. CsSAM identified 1,559 transcripts differentially expressed (all down-regulated) between pre- and post-methacholine in eosinophils at a false discovery cutoff of 10%. Notable changes included the GOLGA5 and METTL2B genes and the protein ubiquitination and CCR3 pathways. Conclusions: We demonstrated significant changes in the peripheral blood eosinophil-specific transcriptome of asthmatics two hours after methacholine challenge. CCR3 and protein ubiquitination pathways are both significantly down-regulated. äTranscriptional Changes of Blood Eosinophils After Methacholine Inhala(epmc).pdf DeepDyve Pubget Overpricing