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2015 ; 15
(6
): 14356-69
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Application of Cavity Enhanced Absorption Spectroscopy to the Detection of Nitric
Oxide, Carbonyl Sulphide, and Ethane--Breath Biomarkers of Serious Diseases
#MMPMID26091398
Wojtas J
Sensors (Basel)
2015[Jun]; 15
(6
): 14356-69
PMID26091398
show ga
The paper presents one of the laser absorption spectroscopy techniques as an
effective tool for sensitive analysis of trace gas species in human breath.
Characterization of nitric oxide, carbonyl sulphide and ethane, and the selection
of their absorption lines are described. Experiments with some biomarkers showed
that detection of pathogenic changes at the molecular level is possible using
this technique. Thanks to cavity enhanced spectroscopy application, detection
limits at the ppb-level and short measurements time (<3 s) were achieved.
Absorption lines of reference samples of the selected volatile biomarkers were
probed using a distributed feedback quantum cascade laser and a tunable laser
system consisting of an optical parametric oscillator and difference frequency
generator. Setup using the first source provided a detection limit of 30 ppb for
nitric oxide and 250 ppb for carbonyl sulphide. During experiments employing a
second laser, detection limits of 0.9 ppb and 0.3 ppb were obtained for carbonyl
sulphide and ethane, respectively. The conducted experiments show that this type
of diagnosis would significantly increase chances for effective therapy of some
diseases. Additionally, it offers non-invasive and real time measurements, high
sensitivity and selectivity as well as minimizing discomfort for patients. For
that reason, such sensors can be used in screening for early detection of serious
diseases.