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10.1186/s13072-015-0017-5 http://scihub22266oqcxt.onion/10.1186/s13072-015-0017-5 C4507313!4507313!26195988     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Epigenetics+Chromatin 2015 ; 8 (ä): ä Nephropedia Template TP {{tp|p=26195988|t=2015. lobChIP: from cells to sequencing ready ChIP libraries in a single day |pdf=|usr=}}{{26195988}} gab.com Text lobChIP: from cells to sequencing ready ChIP libraries in a single day JO: Epigenetics Chromatin http://www.kidney.de/pget.php?&tw=1&pmid=26195988 #FOAvip Background: ChIP-seq is the method of choice for genome-wide studies of protein?DNA interactions. We describe a new method for ChIP-seq sample preparation, termed lobChIP, where the library reactions are performed on cross-linked ChIP fragments captured on beads. Results: The lobChIP method was found both to reduce time and cost and to simplify the processing of many samples in parallel. lobChIP has an early incorporation of barcoded sequencing adaptors that minimizes the risk of sample cross-contamination and can lead to reduced amount of adaptor dimers in the sequencing libraries, while allowing for direct decross-linking and amplification of the sample. Conclusions: With results for histone modifications and transcription factors, we show that lobChIP performs equal to or better than standard protocols and that it makes it possible to go from cells to sequencing ready libraries within a single day. Electronic supplementary material: The online version of this article (doi:10.1186/s13072-015-0017-5) contains supplementary material, which is available to authorized users. Twit Text FOAVip lobChIP: from cells to sequencing ready ChIP libraries in a single day ????Epigenetics Chromatin http://www.kidney.de/pget.php?&tw=1&pmid=26195988 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26195988 #FOAvip English Wikipedia <ref>{{Cite pmid|26195988}}</ref> lobChIP: from cells to sequencing ready ChIP libraries in a single day #MMPMID26195988 Wallerman O; Nord H; Bysani M; Borghini L; Wadelius C Epigenetics Chromatin 2015[]; 8 (ä): ä PMID26195988show ga Background: ChIP-seq is the method of choice for genome-wide studies of protein?DNA interactions. We describe a new method for ChIP-seq sample preparation, termed lobChIP, where the library reactions are performed on cross-linked ChIP fragments captured on beads. Results: The lobChIP method was found both to reduce time and cost and to simplify the processing of many samples in parallel. lobChIP has an early incorporation of barcoded sequencing adaptors that minimizes the risk of sample cross-contamination and can lead to reduced amount of adaptor dimers in the sequencing libraries, while allowing for direct decross-linking and amplification of the sample. Conclusions: With results for histone modifications and transcription factors, we show that lobChIP performs equal to or better than standard protocols and that it makes it possible to go from cells to sequencing ready libraries within a single day. Electronic supplementary material: The online version of this article (doi:10.1186/s13072-015-0017-5) contains supplementary material, which is available to authorized users. älobChIP: from cells to sequencing ready ChIP libraries in a single day(epmc).pdf DeepDyve Pubget Overpricing