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10.1074/jbc.M115.646034 http://scihub22266oqcxt.onion/10.1074/jbc.M115.646034 C4505433!4505433 !26013827     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26013827 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       J+Biol+Chem 2015 ; 290 (27 ): 16873-81 Nephropedia Template TP {{tp|p=26013827 |t=2015. Identification and Molecular Mechanisms of the Rapid Tonicity-induced Relocalization of the Aquaporin 4 Channel |pdf=|usr=}}{{26013827 }} gab.com Text Identification and Molecular Mechanisms of the Rapid Tonicity-induced Relocalization of the Aquaporin 4 Channel JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=26013827 #FOAvip The aquaporin family of integral membrane proteins is composed of channels that mediate cellular water flow. Aquaporin 4 (AQP4) is highly expressed in the glial cells of the central nervous system and facilitates the osmotically driven pathological brain swelling associated with stroke and traumatic brain injury. Here we show that AQP4 cell surface expression can be rapidly and reversibly regulated in response to changes of tonicity in primary cortical rat astrocytes and in transfected HEK293 cells. The translocation mechanism involves PKA activation, influx of extracellular calcium, and activation of calmodulin. We identify five putative PKA phosphorylation sites and use site-directed mutagenesis to show that only phosphorylation at one of these sites, serine 276, is necessary for the translocation response. We discuss our findings in the context of the identification of new therapeutic approaches to treating brain edema. Twit Text FOAVip Identification and Molecular Mechanisms of the Rapid Tonicity-induced Relocalization of the Aquaporin 4 Channel ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=26013827 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26013827 #FOAvip English Wikipedia <ref>{{Cite pmid|26013827 }}</ref> Identification and Molecular Mechanisms of the Rapid Tonicity-induced Relocalization of the Aquaporin 4 Channel #MMPMID26013827 Kitchen P ; Day RE ; Taylor LH ; Salman MM ; Bill RM ; Conner MT ; Conner AC J Biol Chem 2015[Jul]; 290 (27 ): 16873-81 PMID26013827 show ga The aquaporin family of integral membrane proteins is composed of channels that mediate cellular water flow. Aquaporin 4 (AQP4) is highly expressed in the glial cells of the central nervous system and facilitates the osmotically driven pathological brain swelling associated with stroke and traumatic brain injury. Here we show that AQP4 cell surface expression can be rapidly and reversibly regulated in response to changes of tonicity in primary cortical rat astrocytes and in transfected HEK293 cells. The translocation mechanism involves PKA activation, influx of extracellular calcium, and activation of calmodulin. We identify five putative PKA phosphorylation sites and use site-directed mutagenesis to show that only phosphorylation at one of these sites, serine 276, is necessary for the translocation response. We discuss our findings in the context of the identification of new therapeutic approaches to treating brain edema. |Amino Acid Motifs [MESH] |Animals [MESH] |Aquaporin 4/chemistry/genetics/*metabolism [MESH] |Astrocytes/metabolism [MESH] |Brain Edema/genetics/*metabolism [MESH] |Calcium/metabolism [MESH] |Calmodulin/metabolism [MESH] |Cells, Cultured [MESH] |Cyclic AMP-Dependent Protein Kinases/metabolism [MESH] |HEK293 Cells [MESH] |Humans [MESH] |Osmotic Pressure [MESH] |Phosphorylation [MESH] |Protein Transport [MESH]Identification and Molecular Mechanisms of the Rapid Tonicity-induced (epmc).pdf DeepDyve Pubget Overpricing