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Rutin ameliorates renal fibrosis and proteinuria in 5/6-nephrectomized rats by
anti-oxidation and inhibiting activation of TGF?1-smad signaling
#MMPMID26191162
Han Y
; Lu JS
; Xu Y
; Zhang L
; Hong BF
Int J Clin Exp Pathol
2015[]; 8
(5
): 4725-34
PMID26191162
show ga
OBJECTIVES: Rutin, a polyphenolic flavonoid, was reported to have beneficial
effect on drug induced nephropathy. The present study aimed to introduce 5/6
nephrectomized rat model to further evaluate its renal protective effect.
METHODS: Adult Wistar rats were induced to develop chronic renal failure through
5/6 nephrectomy (5/6 Nx). After that, animals were treated orally with saline,
rutin at 15 and 45 mg/kg, and losartan (10 mg/kg) daily for 20 weeks;
sham-operated animals were also involved as control. After treatment for 8 and 20
weeks, blood and urine samples were collected for biochemical examination; all
the kidney remnants were collected for histological examination. The protein
levels of TGF-?1, smad2 and phosphorylated-smad2 (p-smad2) in kidney were
measured. Immunohistochemistry was used to analyze the expression of TGF-?1,
fibronectin and collagen IV in kidney tissues. RESULTS: Results suggested that
rutin could reduce the proteinurea, blood urine nitrogen and blood creatinine in
5/6 Nx animals significantly, as well as oxidation stress in the kidney. By
histological examination, rutin administration alleviated glomerular sclerosis
scores and tubulointerstitial injuries in a dose-dependent manner (P<0.01).
Immunohistochemistry also suggested rutin could reduce the expression of TGF-?1,
fibronectin and collagen IV in kidney tissues. By western blot, we found the
rutin could reduce the TGF-?1, p-smad2 expression in the kidney tissues of rats.
CONCLUSIONS: This study suggests that the rutin can improve renal function in 5/6
Nx rats effectively. Its effect may be due to its anti-oxidation and inhibiting
TGF?1-Smad signaling.
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