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Podocyte-associated mRNA profiles in kidney tissue and in urine of patients with
active lupus nephritis
#MMPMID26191151
dos Santos M
; Bringhenti RN
; Rodrigues PG
; do Nascimento JF
; Pereira SV
; Zancan R
; Monticielo OA
; Gasparin AA
; de Castro WP
; Veronese FV
Int J Clin Exp Pathol
2015[]; 8
(5
): 4600-13
PMID26191151
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AIM: Glomerular deposition of immune complexes and inflammation induce podocyte
injury in lupus nephritis (LN). This study hypothesized that the severity of the
histological lesions of LN affects podocyte-associated mRNAs profiles in kidney
tissue and in urine. METHODS: Thirty-three patients with LN were grouped
according to the presence of mild mesangial (classes I and II) or
moderate-to-severe immune complex deposition, proliferation and/or inflammation
(classes III, IV and V) in kidney biopsy. Tissue and urine mRNA of nephrin,
podocin, podocalyxin, ?-actinin-4, transient receptor potential cation channel 6,
and of growth factors VEGF-A and TGF-?1 and the transcription factor FOXP3 were
measured using real time polymerase chain reaction. These mRNAs were correlated
with histological severity of LN, extent of glomerular immune deposits, and
tissue infiltrating cells. RESULTS: Podocyte-associated mRNAs were inhibited in
renal tissue of patients with LN irrespective of histological class when compared
to controls. However, significantly higher expression of podocyte mRNAs in urine,
including those of growth factors and FOXP3, were found in patients with
moderate-to-severe nephritis, mostly in class III and IV proliferative forms. The
number of invading CD8+ T cells, B cells and macrophages correlated positively
with urine podocyte-associated mRNAs. Urine podocyte mRNAs also correlated with
proteinuria. CONCLUSIONS: Inhibition of podocyte-associated mRNAs in kidney
tissue suggests that podocyte injury occurs regardless of class severity of LN.
Increased urinary excretion of podocyte mRNAs, mostly in patients with
moderate-to-severe lesions, may reflect a greater burden of glomerular damage
with detachment of podocytes into the urine.
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