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How a Small Double-Stranded Trick Can Mislead Sanger Sequencing #MMPMID26190945
Curci PL; Sonnante G
J Biomol Tech 2015[Sep]; 26 (3): 80-2 PMID26190945show ga
Notwithstanding the arrival of ?third-generation sequencing,? Sanger sequencing, developed in 1980, is still the most accurate and used method for sequencing, although on a smaller scale. It is a powerful resource for studying sequences and discovering polymorphisms and genes, as well as regulatory elements. There has already been described a wide range of possible problems with this very sensitive and accurate technology. Here, we show that a specific event, related to genomes rich in repetitive sequences, can mislead operators working with Sanger sequencing.