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10.7171/jbt.15-2603-004

http://scihub22266oqcxt.onion/10.7171/jbt.15-2603-004
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C4502656!4502656!26190945
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suck abstract from ncbi


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pmid26190945      J+Biomol+Tech 2015 ; 26 (3): 80-2
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  • How a Small Double-Stranded Trick Can Mislead Sanger Sequencing #MMPMID26190945
  • Curci PL; Sonnante G
  • J Biomol Tech 2015[Sep]; 26 (3): 80-2 PMID26190945show ga
  • Notwithstanding the arrival of ?third-generation sequencing,? Sanger sequencing, developed in 1980, is still the most accurate and used method for sequencing, although on a smaller scale. It is a powerful resource for studying sequences and discovering polymorphisms and genes, as well as regulatory elements. There has already been described a wide range of possible problems with this very sensitive and accurate technology. Here, we show that a specific event, related to genomes rich in repetitive sequences, can mislead operators working with Sanger sequencing.
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