Gold nanoparticles promote osteogenic differentiation in human adipose-derived
mesenchymal stem cells through the Wnt/?-catenin signaling pathway
#MMPMID26185441
Choi SY
; Song MS
; Ryu PD
; Lam AT
; Joo SW
; Lee SY
Int J Nanomedicine
2015[]; 10
(?): 4383-92
PMID26185441
show ga
Gold nanoparticles (AuNPs) are attractive materials for use in biomedicine due to
their physical properties. Increasing evidence suggests that several
nanoparticles induce the differentiation of human mesenchymal stem cells into
osteoblasts and adipocytes. In this study, we hypothesized that
chitosan-conjugated AuNPs promote the osteogenic differentiation of human
adipose-derived mesenchymal stem cells. For the evaluation of osteogenic
differentiation, alizarin red staining, an alamarBlue(®) assay, and a
quantitative real-time polymerase chain reaction analysis were performed. In
order to examine specific signaling pathways, immunofluorescence and a western
blotting assay were performed. Our results demonstrate that chitosan-conjugated
AuNPs increase the deposition of calcium content and the expression of marker
genes related to osteogenic differentiation in human adipose-derived mesenchymal
stem cells at nontoxic concentrations. These results indicate that
chitosan-conjugated AuNPs promote osteogenesis through the Wnt/?-catenin
signaling pathway. Therefore, chitosan-conjugated AuNPs can be used as a reagent
for promoting bone formation.
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