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10.1016/j.molcel.2015.01.027 http://scihub22266oqcxt.onion/10.1016/j.molcel.2015.01.027 C4500162!4500162!25728770     free     free     free       Mol+Cell 2015 ; 58 (1): 147-56 Nephropedia Template TP {{tp|p=25728770|t=2015. Bursty gene expression in the intact mammalian liver |pdf=|usr=}}{{25728770}} gab.com Text Bursty gene expression in the intact mammalian liver JO: Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=25728770 #FOAvip Bursts of nascent mRNA have been shown to lead to substantial cell-cell variation in unicellular organisms, facilitating diverse responses to environmental challenges. It is unknown whether similar bursts and gene-expression noise occur in mammalian tissues. To address this, we combine single molecule transcript counting with dual-color labeling and quantification of nascent mRNA to characterize promoter states, transcription rates and transcript lifetimes in the intact mouse liver. We find that liver gene expression is highly bursty, with promoters stochastically switching between transcriptionally active and inactive states. Promoters of genes with short mRNA lifetimes are active longer, facilitating rapid response while reducing burst-associated noise. Moreover, polyploid hepatocytes exhibit less noise than diploid hepatocytes, suggesting a possible benefit to liver polyploidy. Thus temporal averaging and liver polyploidy dampen the intrinsic variability associated with transcriptional bursts. Our approach can be used to study transcriptional bursting in diverse mammalian tissues. Twit Text FOAVip Bursty gene expression in the intact mammalian liver ????Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=25728770 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25728770 #FOAvip English Wikipedia <ref>{{Cite pmid|25728770}}</ref> Bursty gene expression in the intact mammalian liver #MMPMID25728770 Halpern KB; Tanami S; Landen S; Chapal M; Szlak L; Hutzler A; Nizhberg A; Itzkovitz S Mol Cell 2015[Apr]; 58 (1): 147-56 PMID25728770show ga Bursts of nascent mRNA have been shown to lead to substantial cell-cell variation in unicellular organisms, facilitating diverse responses to environmental challenges. It is unknown whether similar bursts and gene-expression noise occur in mammalian tissues. To address this, we combine single molecule transcript counting with dual-color labeling and quantification of nascent mRNA to characterize promoter states, transcription rates and transcript lifetimes in the intact mouse liver. We find that liver gene expression is highly bursty, with promoters stochastically switching between transcriptionally active and inactive states. Promoters of genes with short mRNA lifetimes are active longer, facilitating rapid response while reducing burst-associated noise. Moreover, polyploid hepatocytes exhibit less noise than diploid hepatocytes, suggesting a possible benefit to liver polyploidy. Thus temporal averaging and liver polyploidy dampen the intrinsic variability associated with transcriptional bursts. Our approach can be used to study transcriptional bursting in diverse mammalian tissues. äBursty gene expression in the intact mammalian liver (epmc).pdf DeepDyve Pubget Overpricing