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2015 ; 2015
(ä): 257019
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Angiogenic Potential of Human Neonatal Foreskin Stromal Cells in the Chick Embryo
Chorioallantoic Membrane Model
#MMPMID26221144
Vishnubalaji R
; Atteya M
; Al-Nbaheen M
; Oreffo RO
; Aldahmash A
; Alajez NM
Stem Cells Int
2015[]; 2015
(ä): 257019
PMID26221144
show ga
Several studies have demonstrated the multipotentiality of human neonatal
foreskin stromal cells (hNSSCs) as being able to differentiate into adipocytes
and osteoblasts and potentially other cell types. Recently, we demonstrated that
hNSSCs play a role during in vitro angiogenesis and appear to possess a capacity
to differentiate into endothelial-like cells; however, their angiogenic potential
within an ex vivo environment remains unclear. Current study shows hNSSCs to
display significant migration potential in the undifferentiated state and high
responsiveness in the in vitro wound healing scratch assay. When hNSSCs were
seeded onto the top of the CAM, human von Willebrand factor (hVWF), CD31, smooth
muscle actin (SMA), and factor XIIIa positive cells were observed in the chick
endothelium. CAMs transplanted with endothelial-differentiated hNSSCs displayed a
higher number of blood vessels containing hNSSCs compared to CAMs transplanted
with undifferentiated hNSSCs. Interestingly, undifferentiated hNSSCs showed a
propensity to differentiate towards ectoderm with indication of epidermal
formation with cells positive for CD1a, CK5/6, CK19, FXIIIa, and S-100 cells,
which warrant further investigation. Our findings imply a potential angiogenic
role for hNSSCs ex vivo in the differentiated and undifferentiated state, with
potential contribution to blood vessel formation and potential application in
tissue regeneration and vascularization.