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10.1021/acs.nanolett.5b00852

http://scihub22266oqcxt.onion/10.1021/acs.nanolett.5b00852
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C4498449!4498449!26053894
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suck abstract from ncbi


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pmid26053894      Nano+Lett 2015 ; 15 (7): 4467-71
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  • General Strategy to Introduce pH-Induced Allostery in DNA-Based Receptors to Achieve Controlled Release of Ligands #MMPMID26053894
  • Porchetta A; Idili A; Vallée-Bélisle A; Ricci F
  • Nano Lett 2015[Jul]; 15 (7): 4467-71 PMID26053894show ga
  • Inspired by naturally occurring pH-regulated receptors, here we propose a rational approach to introduce pH-induced allostery into a wide range of DNA-based receptors. To demonstrate this we re-engineered two model DNA-based probes, a molecular beacon and a cocaine-binding aptamer, by introducing in their sequence a pH-dependent domain. We demonstrate here that we can finely tune the affinity of these model receptors and control the load/release of their specific target molecule by a simple pH change.
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