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2015 ; 10
(6
): 904-8
Nephropedia Template TP
gab.com Text
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12 hours after cerebral ischemia is the optimal time for bone marrow mesenchymal
stem cell transplantation
#MMPMID26199606
Hosseini SM
; Farahmandnia M
; Razi Z
; Delavarifar S
; Shakibajahromi B
Neural Regen Res
2015[Jun]; 10
(6
): 904-8
PMID26199606
show ga
Cell therapy using stem cell transplantation against cerebral ischemia has been
reported. However, it remains controversial regarding the optimal time for cell
transplantation and the transplantation route. Rat models of cerebral ischemia
were established by occlusion of the middle cerebral artery. At 1, 12 hours, 1,
3, 5 and 7 days after cerebral ischemia, bone marrow mesenchymal stem cells were
injected via the tail vein. At 28 days after cerebral ischemia, rat neurological
function was evaluated using a 6-point grading scale and the pathological change
of ischemic cerebral tissue was observed by hematoxylin-eosin staining. Under the
fluorescence microscope, the migration of bone marrow mesenchymal stem cells was
examined by PKH labeling. Caspase-3 activity was measured using
spectrophotometry. The optimal neurological function recovery, lowest degree of
ischemic cerebral damage, greatest number of bone marrow mesenchymal stem cells
migrating to peri-ischemic area, and lowest caspase-3 activity in the ischemic
cerebral tissue were observed in rats that underwent bone marrow mesenchymal stem
cell transplantation at 12 hours after cerebral ischemia. These findings suggest
that 12 hours after cerebral ischemia is the optimal time for tail vein injection
of bone marrow mesenchymal stem cell transplantation against cerebral ischemia,
and the strongest neuroprotective effect of this cell therapy appears at this
time.