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10.1038/srep12022 http://scihub22266oqcxt.onion/10.1038/srep12022 C4496662!4496662!26156563     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2015 ; 5 (ä): ä Nephropedia Template TP {{tp|p=26156563|t=2015. DNA-dependent targeting of cell nuclei by a lupus autoantibody |pdf=|usr=}}{{26156563}} gab.com Text DNA-dependent targeting of cell nuclei by a lupus autoantibody JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26156563 #FOAvip A nuclear-penetrating lupus anti-DNA autoantibody, 3E10, has been found to inhibit DNA repair and selectively kill certain cancer cells that are highly vulnerable to DNA damage. In addition, a 3E10 single chain variable fragment (scFv) has been developed for use as a delivery vehicle to carry therapeutic cargo proteins into cell nuclei. A greater understanding of the mechanism by which 3E10 penetrates cell nuclei is needed to help determine the scope of its potential therapeutic applications. Here we show that the presence of extracellular DNA significantly enhances the nuclear uptake of 3E10 scFv. In addition, we find that 3E10 scFv preferentially localizes into tumor cell nuclei in vivo, likely due to increased DNA in the local environment released from ischemic and necrotic regions of tumor. These data provide insight into the mechanism of nuclear penetration by 3E10 and demonstrate the potential for use of 3E10 in therapeutic approaches to diseases ranging from malignancy to ischemic conditions such as stroke. Twit Text FOAVip DNA-dependent targeting of cell nuclei by a lupus autoantibody ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26156563 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26156563 #FOAvip English Wikipedia <ref>{{Cite pmid|26156563}}</ref> DNA-dependent targeting of cell nuclei by a lupus autoantibody #MMPMID26156563 Weisbart RH; Chan G; Jordaan G; Noble PW; Liu Y; Glazer PM; Nishimura RN; Hansen JE Sci Rep 2015[]; 5 (ä): ä PMID26156563show ga A nuclear-penetrating lupus anti-DNA autoantibody, 3E10, has been found to inhibit DNA repair and selectively kill certain cancer cells that are highly vulnerable to DNA damage. In addition, a 3E10 single chain variable fragment (scFv) has been developed for use as a delivery vehicle to carry therapeutic cargo proteins into cell nuclei. A greater understanding of the mechanism by which 3E10 penetrates cell nuclei is needed to help determine the scope of its potential therapeutic applications. Here we show that the presence of extracellular DNA significantly enhances the nuclear uptake of 3E10 scFv. In addition, we find that 3E10 scFv preferentially localizes into tumor cell nuclei in vivo, likely due to increased DNA in the local environment released from ischemic and necrotic regions of tumor. These data provide insight into the mechanism of nuclear penetration by 3E10 and demonstrate the potential for use of 3E10 in therapeutic approaches to diseases ranging from malignancy to ischemic conditions such as stroke. äDNA-dependent targeting of cell nuclei by a lupus autoantibody (epmc).pdf DeepDyve Pubget Overpricing