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10.18632/oncotarget.3171 http://scihub22266oqcxt.onion/10.18632/oncotarget.3171 C4496413!4496413 !25846752     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25846752 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Oncotarget 2015 ; 6 (12 ): 9999-10015 Nephropedia Template TP {{tp|p=25846752 |t=2015. Aspirin counteracts cancer stem cell features, desmoplasia and gemcitabine resistance in pancreatic cancer |pdf=|usr=}}{{25846752 }} gab.com Text Aspirin counteracts cancer stem cell features, desmoplasia and gemcitabine resistance in pancreatic cancer JO: Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=25846752 #FOAvip Pancreatic ductal adenocarcinoma (PDA) is characterized by an extremely poor prognosis. An inflammatory microenvironment triggers the pronounced desmoplasia, the selection of cancer stem-like cells (CSCs) and therapy resistance. The anti-inflammatory drug aspirin is suggested to lower the risk for PDA and to improve the treatment, although available results are conflicting and the effect of aspirin to CSC characteristics and desmoplasia in PDA has not yet been investigated. We characterized the influence of aspirin on CSC features, stromal reactions and gemcitabine resistance. Four established and 3 primary PDA cell lines, non-malignant cells, 3 patient tumor-derived CSC-enriched spheroidal cultures and tissues from patients who did or did not receive aspirin before surgery were analyzed using MTT assays, flow cytometry, colony and spheroid formation assays, Western blot analysis, antibody protein arrays, electrophoretic mobility shift assays (EMSAs), immunohistochemistry and in vivo xenotransplantation. Aspirin significantly induced apoptosis and reduced the viability, self-renewal potential, and expression of proteins involved in inflammation and stem cell signaling. Aspirin also reduced the growth and invasion of tumors in vivo, and it significantly prolonged the survival of mice with orthotopic pancreatic xenografts in combination with gemcitabine. This was associated with a decreased expression of markers for progression, inflammation and desmoplasia. These findings were confirmed in tissue samples obtained from patients who had or had not taken aspirin before surgery. Importantly, aspirin sensitized cells that were resistant to gemcitabine and thereby enhanced the therapeutic efficacy. Aspirin showed no obvious toxic effects on normal cells, chick embryos or mice. These results highlight aspirin as an effective, inexpensive and well-tolerated co-treatment to target inflammation, desmoplasia and CSC features PDA. Twit Text FOAVip Aspirin counteracts cancer stem cell features, desmoplasia and gemcitabine resistance in pancreatic cancer ????Oncotarget http://www.kidney.de/pget.php?&tw=1&pmid=25846752 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25846752 #FOAvip English Wikipedia <ref>{{Cite pmid|25846752 }}</ref> Aspirin counteracts cancer stem cell features, desmoplasia and gemcitabine resistance in pancreatic cancer #MMPMID25846752 Zhang Y ; Liu L ; Fan P ; Bauer N ; Gladkich J ; Ryschich E ; Bazhin AV ; Giese NA ; Strobel O ; Hackert T ; Hinz U ; Gross W ; Fortunato F ; Herr I Oncotarget 2015[Apr]; 6 (12 ): 9999-10015 PMID25846752 show ga Pancreatic ductal adenocarcinoma (PDA) is characterized by an extremely poor prognosis. An inflammatory microenvironment triggers the pronounced desmoplasia, the selection of cancer stem-like cells (CSCs) and therapy resistance. The anti-inflammatory drug aspirin is suggested to lower the risk for PDA and to improve the treatment, although available results are conflicting and the effect of aspirin to CSC characteristics and desmoplasia in PDA has not yet been investigated. We characterized the influence of aspirin on CSC features, stromal reactions and gemcitabine resistance. Four established and 3 primary PDA cell lines, non-malignant cells, 3 patient tumor-derived CSC-enriched spheroidal cultures and tissues from patients who did or did not receive aspirin before surgery were analyzed using MTT assays, flow cytometry, colony and spheroid formation assays, Western blot analysis, antibody protein arrays, electrophoretic mobility shift assays (EMSAs), immunohistochemistry and in vivo xenotransplantation. Aspirin significantly induced apoptosis and reduced the viability, self-renewal potential, and expression of proteins involved in inflammation and stem cell signaling. Aspirin also reduced the growth and invasion of tumors in vivo, and it significantly prolonged the survival of mice with orthotopic pancreatic xenografts in combination with gemcitabine. This was associated with a decreased expression of markers for progression, inflammation and desmoplasia. These findings were confirmed in tissue samples obtained from patients who had or had not taken aspirin before surgery. Importantly, aspirin sensitized cells that were resistant to gemcitabine and thereby enhanced the therapeutic efficacy. Aspirin showed no obvious toxic effects on normal cells, chick embryos or mice. These results highlight aspirin as an effective, inexpensive and well-tolerated co-treatment to target inflammation, desmoplasia and CSC features PDA. |Animals [MESH] |Antineoplastic Combined Chemotherapy Protocols/*pharmacology [MESH] |Aspirin/administration & dosage/*pharmacology [MESH] |Biomarkers, Tumor/metabolism [MESH] |Carcinoma, Pancreatic Ductal/*drug therapy/pathology [MESH] |Cell Line, Tumor [MESH] |Chick Embryo [MESH] |Deoxycytidine/administration & dosage/*analogs & derivatives/pharmacology [MESH] |Drug Resistance, Neoplasm [MESH] |Drug Synergism [MESH] |Gemcitabine [MESH] |Humans [MESH] |Male [MESH] |Mice [MESH] |Mice, Nude [MESH] |Neoplastic Stem Cells/*drug effects/pathology [MESH] |Pancreatic Neoplasms/*drug therapy/pathology [MESH] |Signal Transduction [MESH]Aspirin counteracts cancer stem cell features, desmoplasia and gemcita(epmc).pdf DeepDyve Pubget Overpricing