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10.1038/srep12030 http://scihub22266oqcxt.onion/10.1038/srep12030 C4495553!4495553!26153530     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2015 ; 5 (ä): ä Nephropedia Template TP {{tp|p=26153530|t=2015. Study of Protein Expresion in Peri-Infarct Tissue after Cerebral Ischemia |pdf=|usr=}}{{26153530}} gab.com Text Study of Protein Expresion in Peri-Infarct Tissue after Cerebral Ischemia JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26153530 #FOAvip In this work, we report our study of protein expression in rat peri-infarct tissue, 48?h after the induction of permanent focal cerebral ischemia. Two proteomic approaches, gel electrophoresis with mass spectrometry and combined fractional diagonal chromatography (COFRADIC), were performed using tissue samples from the periphery of the induced cerebral ischemic lesions, using tissue from the contra-lateral hemisphere as a control. Several protein spots (3408) were identified by gel electrophoresis, and 11 showed significant differences in expression between peri-infarct and contra-lateral tissues (at least 3-fold, p?<?0.05). Using COFRADIC, 5412 proteins were identified, with 72 showing a difference in expression. Apart from blood-related proteins (such as serum albumin), both techniques showed that the 70?kDa family of heat shock proteins were highly expressed in the peri-infarct tissue. Further studies by 1D and 2D western blotting and immunohistochemistry revealed that only one member of this family (the inducible form, HSP72 or HSP70i) is specifically expressed by the peri-infarct tissue, while the majority of this family (the constitutive form, HSC70 or HSP70c) is expressed in the whole brain. Our data support that HSP72 is a suitable biomarker of peri-infarct tissue in the ischemic brain. Twit Text FOAVip Study of Protein Expresion in Peri-Infarct Tissue after Cerebral Ischemia ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26153530 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26153530 #FOAvip English Wikipedia <ref>{{Cite pmid|26153530}}</ref> Study of Protein Expresion in Peri-Infarct Tissue after Cerebral Ischemia #MMPMID26153530 Brea D; Agulla J; Staes A; Gevaert K; Campos F; Sobrino T; Blanco M; Dávalos A; Castillo J; Ramos-Cabrer P Sci Rep 2015[]; 5 (ä): ä PMID26153530show ga In this work, we report our study of protein expression in rat peri-infarct tissue, 48?h after the induction of permanent focal cerebral ischemia. Two proteomic approaches, gel electrophoresis with mass spectrometry and combined fractional diagonal chromatography (COFRADIC), were performed using tissue samples from the periphery of the induced cerebral ischemic lesions, using tissue from the contra-lateral hemisphere as a control. Several protein spots (3408) were identified by gel electrophoresis, and 11 showed significant differences in expression between peri-infarct and contra-lateral tissues (at least 3-fold, p? äStudy of Protein Expresion in Peri-Infarct Tissue after Cerebral Ische(epmc).pdf DeepDyve Pubget Overpricing