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2015 ; 10
(11
): 1793-802
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Engraftment of embryonic stem cells and differentiated progeny by host
conditioning with total lymphoid irradiation and regulatory T cells
#MMPMID25801020
Pan Y
; Leveson-Gower DB
; de Almeida PE
; Pierini A
; Baker J
; Florek M
; Nishikii H
; Kim BS
; Ke R
; Wu JC
; Negrin RS
Cell Rep
2015[Mar]; 10
(11
): 1793-802
PMID25801020
show ga
Embryonic stem cells (ESCs) hold promise for the treatment of many medical
conditions; however, their utility is limited by immune rejection. The objective
of our study is to establish tolerance or promote engraftment of transplanted
ESCs as well as mature cell populations derived from ESCs. Luciferase
(luc(+))-expressing ESCs were utilized to monitor the survival of the ESCs and
differentiated progeny in living recipients. Allogeneic recipients conditioned
with fractioned total lymphoid irradiation (TLI) and anti-thymocyte serum (ATS)
or TLI plus regulatory T cells (T(reg)) promoted engraftment of ESC allografts
after transplantation. Following these treatments, the engraftment of
transplanted terminally differentiated endothelial cells derived from ESCs was
also significantly enhanced. Our findings provide clinically translatable
strategies of inducing tolerance to adoptively transferred ESCs for cell
replacement therapy of medical disorders.