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10.1038/nprot.2015.011 http://scihub22266oqcxt.onion/10.1038/nprot.2015.011 C4494653!4494653!25692985     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Protoc 2015 ; 10 (3): 484-94 Nephropedia Template TP {{tp|p=25692985|t=2015. Supercooling Preservation Of The Rat Liver For Transplantation |pdf=|usr=}}{{25692985}} gab.com Text Supercooling Preservation Of The Rat Liver For Transplantation JO: Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=25692985 #FOAvip The current standard for liver preservation is limited in duration. Employing a novel subzero preservation technique that includes supercooling and machine perfusion can significantly improve preservation and prolong storage times. By loading rat livers with cryoprotectants to prevent both intra- and extracellular ice formation and protect against hypothermic injury, livers can be cooled to ?6 °C without freezing and kept viable for up to 96 hours. Here, we describe the procedures of loading cryoprotectants by means of subnormothermic machine perfusion (SNMP), controlled cooling to a supercooled state, followed by SNMP recovery and orthotopic liver transplantation. Twit Text FOAVip Supercooling Preservation Of The Rat Liver For Transplantation ????Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=25692985 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25692985 #FOAvip English Wikipedia <ref>{{Cite pmid|25692985}}</ref> Supercooling Preservation Of The Rat Liver For Transplantation #MMPMID25692985 Bruinsma BG; Berendsen TA; Izamis ML; Yeh H; Yarmush ML; Uygun K Nat Protoc 2015[Mar]; 10 (3): 484-94 PMID25692985show ga The current standard for liver preservation is limited in duration. Employing a novel subzero preservation technique that includes supercooling and machine perfusion can significantly improve preservation and prolong storage times. By loading rat livers with cryoprotectants to prevent both intra- and extracellular ice formation and protect against hypothermic injury, livers can be cooled to ?6 °C without freezing and kept viable for up to 96 hours. Here, we describe the procedures of loading cryoprotectants by means of subnormothermic machine perfusion (SNMP), controlled cooling to a supercooled state, followed by SNMP recovery and orthotopic liver transplantation. äSupercooling Preservation Of The Rat Liver For Transplantation (epmc).pdf DeepDyve Pubget Overpricing