Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1038/nbt.3055 http://scihub22266oqcxt.onion/10.1038/nbt.3055 C4492112!4492112!25326897     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Biotechnol 2015 ; 33 (1): 102-6 Nephropedia Template TP {{tp|p=25326897|t=2015. In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9 |pdf=|usr=}}{{25326897}} gab.com Text In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9 JO: Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=25326897 #FOAvip Probing gene function in the mammalian brain can be greatly assisted with methods to manipulate the genome of neurons in vivo. The clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease (Cas)9 from Streptococcus pyogenes (SpCas9)1 can be used to edit single or multiple genes in replicating eukaryotic cells, resulting in frame-shifting insertion/deletion (indel) mutations and subsequent protein depletion. Here, we delivered SpCas9 and guide RNAs using adeno-associated viral (AAV) vectors to target single (Mecp2) as well as multiple genes (Dnmt1, Dnmt3a and Dnmt3b) in the adult mouse brain in vivo. We characterized the effects of genome modifications in postmitotic neurons using biochemical, genetic, electrophysiological and behavioral readouts. Our results demonstrate that AAV-mediated SpCas9 genome editing can enable reverse genetic studies of gene function in the brain. Twit Text FOAVip In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9 ????Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=25326897 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25326897 #FOAvip English Wikipedia <ref>{{Cite pmid|25326897}}</ref> In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9 #MMPMID25326897 Swiech L; Heidenreich M; Banerjee A; Habib N; Li Y; Trombetta J; Sur M; Zhang F Nat Biotechnol 2015[Jan]; 33 (1): 102-6 PMID25326897show ga Probing gene function in the mammalian brain can be greatly assisted with methods to manipulate the genome of neurons in vivo. The clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease (Cas)9 from Streptococcus pyogenes (SpCas9)1 can be used to edit single or multiple genes in replicating eukaryotic cells, resulting in frame-shifting insertion/deletion (indel) mutations and subsequent protein depletion. Here, we delivered SpCas9 and guide RNAs using adeno-associated viral (AAV) vectors to target single (Mecp2) as well as multiple genes (Dnmt1, Dnmt3a and Dnmt3b) in the adult mouse brain in vivo. We characterized the effects of genome modifications in postmitotic neurons using biochemical, genetic, electrophysiological and behavioral readouts. Our results demonstrate that AAV-mediated SpCas9 genome editing can enable reverse genetic studies of gene function in the brain. äIn vivo interrogation of gene function in the mammalian brain using CR(epmc).pdf DeepDyve Pubget Overpricing