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10.1073/pnas.1508525112 http://scihub22266oqcxt.onion/10.1073/pnas.1508525112 C4491743!4491743!26080436     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proc+Natl+Acad+Sci+U+S+A 2015 ; 112 (26): 8076-81 Nephropedia Template TP {{tp|p=26080436|t=2015. CRISPR-based screening of genomic island excision events in bacteria |pdf=|usr=}}{{26080436}} gab.com Text CRISPR-based screening of genomic island excision events in bacteria JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=26080436 #FOAvip The development of Clustered regularly interspaced short palindromic repeats (CRISPR)?CRISPR-associated genes (CAS)?based technology for targeted genome editing has revolutionized molecular biology approaches, but significant and outstanding gaps exist for applications in bacteria, the native hosts of these adaptive immune systems. This study shows that CRISPR-Cas systems can be directed to target and delete genomic islands that are flanked by insertion-sequence elements and devoid of essential genes. Naturally occurring minor subpopulations harboring deletions in genomic islands were identified and readily isolated using CRISPR-Cas screening. Promising applications of this approach can define minimal bacterial genomes, determine essential genes, and characterize genetically heterogeneous bacterial populations. Twit Text FOAVip CRISPR-based screening of genomic island excision events in bacteria ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=26080436 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26080436 #FOAvip English Wikipedia <ref>{{Cite pmid|26080436}}</ref> CRISPR-based screening of genomic island excision events in bacteria #MMPMID26080436 Selle K; Klaenhammer TR; Barrangou R Proc Natl Acad Sci U S A 2015[Jun]; 112 (26): 8076-81 PMID26080436show ga The development of Clustered regularly interspaced short palindromic repeats (CRISPR)?CRISPR-associated genes (CAS)?based technology for targeted genome editing has revolutionized molecular biology approaches, but significant and outstanding gaps exist for applications in bacteria, the native hosts of these adaptive immune systems. This study shows that CRISPR-Cas systems can be directed to target and delete genomic islands that are flanked by insertion-sequence elements and devoid of essential genes. Naturally occurring minor subpopulations harboring deletions in genomic islands were identified and readily isolated using CRISPR-Cas screening. Promising applications of this approach can define minimal bacterial genomes, determine essential genes, and characterize genetically heterogeneous bacterial populations. äCRISPR-based screening of genomic island excision events in bacteria (epmc).pdf DeepDyve Pubget Overpricing