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Scavenging reactive oxygen species using tempol in the acute phase of renal
ischemia/reperfusion and its effects on kidney oxygenation and nitric oxide
levels
#MMPMID26215821
Aksu U
; Ergin B
; Bezemer R
; Kandil A
; Milstein DM
; Demirci-Tansel C
; Ince C
Intensive Care Med Exp
2015[Dec]; 3
(1
): 57
PMID26215821
show ga
BACKGROUND: Renal ischemia/reperfusion (I/R) injury is commonly seen in kidney
transplantation and affects the allograft survival rates. We aimed to test our
hypothesis that scavenging reactive oxygen species (ROS) with tempol would
protect renal oxygenation and nitric oxide (NO) levels in the acute phase of
renal I/R. METHODS: Rats were randomly divided: (1) no I/R, no tempol; (2) no
I/R, but with tempol; (3) I/R without tempol; and (4) I/R with tempol. I/R was
induced by 30-min clamping of the renal artery. Tempol (200 ?mol/kg/h/i.v) was
administered 15 min prior to I/R. RESULTS: I/R without tempol led to a
significant decrease in renal oxygen delivery and microvascular oxygenation.
Tempol, however, protected renal oxygenation after I/R. At R90, the creatinine
clearance rate was lower in the I/R-subjected group that did not receive tempol
compared to that in the other groups. I/R injury without tempol treatment led to
a significant increase in tissue malondialdehyde levels and a significant
decrease in tissue NO levels. Tempol administration before I/R could prevent
oxidative stress and altered tissue NO levels. CONCLUSIONS: This underscores that
unbalance between oxygen, NO, and ROS forms an important component of the
pathogenesis of I/R-induced AKI and should therefore be taken into account when
designing a prevention/treatment strategy for renal I/R injury in
transplantation.
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