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2015 ; 16
(6
): 12436-53
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gab.com Text
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Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the
Development of Preeclampsia
#MMPMID26042465
Souders CA
; Maynard SE
; Yan J
; Wang Y
; Boatright NK
; Sedan J
; Balyozian D
; Cheslock PS
; Molrine DC
; Simas TA
Int J Mol Sci
2015[Jun]; 16
(6
): 12436-53
PMID26042465
show ga
Angiogenic biomarkers, including soluble fms-like tyrosine kinase 1 (sFlt1), are
thought to be predictors of preeclampsia onset; however, improvement is needed
before a widespread diagnostic test can be utilized. Here we describe the
development and use of diagnostic monoclonal antibodies specific to the two main
splice variants of sFlt1, sFlt1-1 and sFlt1-14. These antibodies were selected
for their sensitivity and specificity to their respective sFlt1 isoform in a
capture ELISA format. Data from this pilot study suggest that sFlt1-1 may be more
predictive of preeclampsia than total sFlt1. It may be possible to improve
current diagnostic platforms if more specific antibodies are utilized.