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10.1038/nnano.2010.23 http://scihub22266oqcxt.onion/10.1038/nnano.2010.23 C4487889!4487889!20228788     free     free     free Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Nanotechnol 2010 ; 5 (4): 291-6 Nephropedia Template TP {{tp|p=20228788|t=2010. Three-dimensional Tissue Culture Based on Magnetic Cell Levitation |pdf=|usr=}}{{20228788}} gab.com Text Three-dimensional Tissue Culture Based on Magnetic Cell Levitation JO: Nat Nanotechnol http://www.kidney.de/pget.php?&tw=1&pmid=20228788 #FOAvip Cell culture is an essential tool for drug discovery, tissue engineering, and stem cell research. Conventional tissue culture produces two-dimensional (2D) cell growth with gene expression, signaling, and morphology that can differ from those in vivo and thus compromise clinical relevancy1?5. Here we report a three-dimensional (3D) culture of cells based on magnetic levitation in the presence of hydrogels containing gold and magnetic iron oxide (MIO) nanoparticles plus filamentous bacteriophage. This methodology allows for control of cell mass geometry and guided, multicellular clustering of different cell types in co-culture through spatial variance of the magnetic field. Moreover, magnetic levitation of human glioblastoma cells demonstrates similar protein expression profiles to those observed in human tumor xenografts. Taken together, these results suggest levitated 3D culture with magnetized phage-based hydrogels more closely recapitulates in vivo protein expression and allows for long-term multi-cellular studies. Twit Text FOAVip Three-dimensional Tissue Culture Based on Magnetic Cell Levitation ????Nat Nanotechnol http://www.kidney.de/pget.php?&tw=1&pmid=20228788 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=20228788 #FOAvip English Wikipedia <ref>{{Cite pmid|20228788}}</ref> Three-dimensional Tissue Culture Based on Magnetic Cell Levitation #MMPMID20228788 Souza GR; Molina JR; Raphael RM; Ozawa MG; Stark DJ; Levin CS; Bronk LF; Ananta JS; Mandelin J; Georgescu MM; Bankson JA; Gelovani JG; Killian TC; Arap W; Pasqualini R Nat Nanotechnol 2010[Apr]; 5 (4): 291-6 PMID20228788show ga Cell culture is an essential tool for drug discovery, tissue engineering, and stem cell research. Conventional tissue culture produces two-dimensional (2D) cell growth with gene expression, signaling, and morphology that can differ from those in vivo and thus compromise clinical relevancy1?5. Here we report a three-dimensional (3D) culture of cells based on magnetic levitation in the presence of hydrogels containing gold and magnetic iron oxide (MIO) nanoparticles plus filamentous bacteriophage. This methodology allows for control of cell mass geometry and guided, multicellular clustering of different cell types in co-culture through spatial variance of the magnetic field. Moreover, magnetic levitation of human glioblastoma cells demonstrates similar protein expression profiles to those observed in human tumor xenografts. Taken together, these results suggest levitated 3D culture with magnetized phage-based hydrogels more closely recapitulates in vivo protein expression and allows for long-term multi-cellular studies. äThree-dimensional Tissue Culture Based on Magnetic Cell Levitation (epmc).pdf DeepDyve Pubget Overpricing