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10.1186/s13059-015-0684-3 http://scihub22266oqcxt.onion/10.1186/s13059-015-0684-3 C4487847!4487847!26047807     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Genome+Biol 2015 ; 16 (1): ä Nephropedia Template TP {{tp|p=26047807|t=2015. Scalable microfluidics for single-cell RNA printing and sequencing |pdf=|usr=}}{{26047807}} gab.com Text Scalable microfluidics for single-cell RNA printing and sequencing JO: Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=26047807 #FOAvip Many important biological questions demand single-cell transcriptomics on a large scale. Hence, new tools are urgently needed for efficient, inexpensive manipulation of RNA from individual cells. We report a simple platform for trapping single-cell lysates in sealed, picoliter microwells capable of printing RNA on glass or capturing RNA on beads. We then develop a scalable technology for genome-wide, single-cell RNA-Seq. Our device generates pooled libraries from hundreds of individual cells with consumable costs of $0.10?$0.20 per cell and includes five lanes for simultaneous experiments. We anticipate that this system will serve as a general platform for single-cell imaging and sequencing.Electronic supplementary material: The online version of this article (doi:10.1186/s13059-015-0684-3) contains supplementary material, which is available to authorized users. Twit Text FOAVip Scalable microfluidics for single-cell RNA printing and sequencing ????Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=26047807 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26047807 #FOAvip English Wikipedia <ref>{{Cite pmid|26047807}}</ref> Scalable microfluidics for single-cell RNA printing and sequencing #MMPMID26047807 Bose S; Wan Z; Carr A; Rizvi AH; Vieira G; Pe?er D; Sims PA Genome Biol 2015[]; 16 (1): ä PMID26047807show ga Many important biological questions demand single-cell transcriptomics on a large scale. Hence, new tools are urgently needed for efficient, inexpensive manipulation of RNA from individual cells. We report a simple platform for trapping single-cell lysates in sealed, picoliter microwells capable of printing RNA on glass or capturing RNA on beads. We then develop a scalable technology for genome-wide, single-cell RNA-Seq. Our device generates pooled libraries from hundreds of individual cells with consumable costs of $0.10?$0.20 per cell and includes five lanes for simultaneous experiments. We anticipate that this system will serve as a general platform for single-cell imaging and sequencing.Electronic supplementary material: The online version of this article (doi:10.1186/s13059-015-0684-3) contains supplementary material, which is available to authorized users. äScalable microfluidics for single-cell RNA printing and sequencing (epmc).pdf DeepDyve Pubget Overpricing