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Allogeneic Bone Marrow Transplant from MRL/MpJ Super-Healer Mice Does Not Improve
Articular Cartilage Repair in the C57Bl/6 Strain
#MMPMID26120841
Leonard CA
; Lee WY
; Tailor P
; Salo PT
; Kubes P
; Krawetz RJ
PLoS One
2015[]; 10
(6
): e0131661
PMID26120841
show ga
BACKGROUND: Articular cartilage has been the focus of multiple strategies to
improve its regenerative/ repair capacity. The Murphy Roths Large (MRL/MpJ)
"super-healer" mouse demonstrates an unusual enhanced regenerative capacity in
many tissues and provides an opportunity to further study endogenous cartilage
repair. The objective of this study was to test whether the super-healer
phenotype could be transferred from MRL/MpJ to non-healer C57Bl/6 mice by
allogeneic bone marrow transplant. METHODOLOGY: The healing of 2mm ear punches
and full thickness cartilage defects was measured 4 and 8 weeks after injury in
control C57Bl/6 and MRL/MpJ "super-healer" mice, and in radiation chimeras
reconstituted with bone marrow from the other mouse strain. Healing was assessed
using ear hole diameter measurement, a 14 point histological scoring scale for
the cartilage defect and an adapted version of the Osteoarthritis Research
Society International scale for assessment of osteoarthritis in mouse knee
joints. PRINCIPAL FINDINGS: Normal and chimeric MRL mice showed significantly
better healing of articular cartilage and ear wounds along with less severe signs
of osteoarthritis after cartilage injury than the control strain. Contrary to our
hypothesis, however, bone marrow transplant from MRL mice did not confer improved
healing on the C57Bl/6 chimeras, either in regards to ear wound healing or
cartilage repair. CONCLUSION AND SIGNIFICANCE: The elusive cellular basis for the
MRL regenerative phenotype still requires additional study and may possibly be
dependent on additional cell types external to the bone marrow.
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