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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Biophotonics
2014 ; 7
(1-2
): 9-22
Nephropedia Template TP
J Biophotonics
2014[Jan]; 7
(1-2
): 9-22
PMID23674234
show ga
Clinical translation of coherent anti-Stokes Raman scattering microscopy is of
great interest because of the advantages of noninvasive label-free imaging, high
sensitivity, and chemical specificity. For this to happen, we have identified and
review the technical barriers that must be overcome. Prior investigations have
developed advanced techniques (features), each of which can be used to
effectively overcome one particular technical barrier. However, the
implementation of one or a small number of these advanced features in previous
attempts for clinical translation has often introduced more tradeoffs than
benefits. In this review, we outline a strategy that would integrate multiple
advanced features to overcome all the technical barriers simultaneously,
effectively reduce tradeoffs, and synergistically optimize CARS microscopy for
clinical translation. The operation of the envisioned system incorporates
coherent Raman micro-spectroscopy for identifying vibrational biomolecular
markers of disease and single-frequency (or hyperspectral) Raman imaging of these
specific biomarkers for real-time in vivo diagnostics and monitoring.