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Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Proc+Natl+Acad+Sci+U+S+A 2015 ; 112 (25): E3282-90 Nephropedia Template TP
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Bioimage analysis of Shigella infection reveals targeting of colonic crypts #MMPMID26056271
Arena ET; Campbell-Valois FX; Tinevez JY; Nigro G; Sachse M; Moya-Nilges M; Nothelfer K; Marteyn B; Shorte SL; Sansonetti PJ
Proc Natl Acad Sci U S A 2015[Jun]; 112 (25): E3282-90 PMID26056271show ga
Shigella spp. are responsible for devastating diarrheal diseases, primarily in children, within underdeveloped countries. Shigella invades the mucosa of the large intestine, causing inflammation and damage to the epithelium. Here, we have measured the progression of Shigella infection in a small animal model of the disease to better understand the mechanism of invasion of the colonic mucosa. The novelty of our approach relies on the tracking of fluorescent bacteria inside the infected tissue at various time points using confocal microscopy and subsequent quantitative bioimage analyses. Our approach is readily applicable to other host?pathogen systems to quantify host?pathogen interactions.