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Cellular and molecular characterization of human cardiac stem cells reveals key
features essential for their function and safety
#MMPMID25867933
Vahdat S
; Mousavi SA
; Omrani G
; Gholampour M
; Sotoodehnejadnematalahi F
; Ghazizadeh Z
; Gharechahi J
; Baharvand H
; Salekdeh GH
; Aghdami N
Stem Cells Dev
2015[Jun]; 24
(12
): 1390-404
PMID25867933
show ga
Cell therapy of heart diseases is emerging as one of the most promising known
treatments in recent years. Transplantation of cardiac stem cells (CSCs) may be
one of the best strategies to cure adult or pediatric heart diseases. As these
patient-derived stem cells need to be isolated from small heart biopsies, it is
important to select the best isolation method and CSC subpopulation with the best
cardiogenic functionality. We employed three different protocols including
c-KIT(+) cell sorting, clonogenic expansion, and explants culture to isolate
c-KIT(+) cells, clonogenic expansion-derived cells (CEDCs), and
cardiosphere-derived cells (CDCs), respectively. Evaluation of isolated CSC
characteristics in vitro and after rat myocardial infarction (MI) model
transplantation revealed that although c-KIT(+) and CDCs had higher MI
regenerative potential, CEDCs had more commitment into cardiomyocytes and needed
lower passages that were essential to reach a definite cell count. Furthermore,
genome-wide expression analysis showed that subsequent passages caused changes in
characteristics of cells, downregulation of cell cycle-related genes, and
upregulation of differentiation and carcinogenic genes, which might lead to
senescence, commitment, and possible tumorigenicity of the cells. Because of
different properties of CSC subpopulations, we suggest that appropriate CSCs
subpopulation should be chosen based on their experimental or clinical use.
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