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2015 ; 126
(ä): 167-202
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The detection and discovery of glycan motifs in biological samples using lectins
and antibodies: new methods and opportunities
#MMPMID25727148
Tang H
; Hsueh P
; Kletter D
; Bern M
; Haab B
Adv Cancer Res
2015[]; 126
(ä): 167-202
PMID25727148
show ga
Recent research has uncovered unexpected ways that glycans contribute to biology,
as well as new strategies for combatting disease using approaches involving
glycans. To make full use of glycans for clinical applications, we need more
detailed information on the location, nature, and dynamics of glycan expression
in vivo. Such studies require the use of specimens acquired directly from
patients. Effective studies of clinical specimens require low-volume assays, high
precision measurements, and the ability to process many samples. Assays using
affinity reagents-lectins and glycan-binding antibodies-can meet these
requirements, but further developments are needed to make the methods routine and
effective. Recent advances in the use of glycan-binding proteins involve improved
determination of specificity using glycan arrays; the availability of databases
for mining and analyzing glycan array data; lectin engineering methods; and the
ability to quantitatively interpret lectin measurements. Here, we describe many
of the challenges and opportunities involved in the application of these new
approaches to the study of biological samples. The new tools hold promise for
developing methods to improve the outcomes of patients afflicted with diseases
characterized by aberrant glycan expression.