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2015 ; 87
(10
): 5095-100
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Nanopipet-Based Liquid-Liquid Interface Probes for the Electrochemical Detection
of Acetylcholine, Tryptamine, and Serotonin via Ionic Transfer
#MMPMID25877788
Colombo ML
; Sweedler JV
; Shen M
Anal Chem
2015[]; 87
(10
): 5095-100
PMID25877788
show ga
A nanoscale interface between two immiscible electrolyte solutions (ITIES)
provides a unique analytical platform for the detection of ionic species of
biological interest such as neurotransmitters and neuromodulators, especially
those that are otherwise difficult to detect directly on a carbon electrode
without electrode modification. We report the detection of acetylcholine,
serotonin, and tryptamine on nanopipet electrode probes with sizes ranging from a
radius of ?7 to 35 nm. The transfer of these analytes across a
1,2-dichloroethane/water interface was studied by cyclic voltammetry and
amperometry. Well-defined sigmoidal voltammograms were observed on the nanopipet
electrodes within the potential window of artificial seawater for acetylcholine
and tryptamine. The half wave transfer potential, E1/2, of acetylcholine,
tryptamine, and serotonin were found to be -0.11, -0.25, and -0.47 V vs
E(1/2,TEA) (term is defined later in experimental), respectively. The detection
was linear in the range of 0.25-6 mM for acetylcholine and of 0.5-10 mM for
tryptamine in artificial seawater. Transfer of serotonin was linear in the range
of 0.15-8 mM in LiCl solution. The limit of detection for serotonin in LiCl on a
radius ?21 nm nanopipet electrode was 77 ?M, for acetylcholine on a radius ?7 nm
nanopipet electrode was 205 ?M, and for tryptamine on a radius ?19 nm nanopipet
electrode was 86 ?M. Nanopipet-supported ITIES probes have great potential to be
used in nanometer spatial resolution measurements for the detection of
neurotransmitters.