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10.1021/ac504151e

http://scihub22266oqcxt.onion/10.1021/ac504151e
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suck abstract from ncbi


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pmid25877788
      Anal+Chem 2015 ; 87 (10 ): 5095-100
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  • Nanopipet-Based Liquid-Liquid Interface Probes for the Electrochemical Detection of Acetylcholine, Tryptamine, and Serotonin via Ionic Transfer #MMPMID25877788
  • Colombo ML ; Sweedler JV ; Shen M
  • Anal Chem 2015[]; 87 (10 ): 5095-100 PMID25877788 show ga
  • A nanoscale interface between two immiscible electrolyte solutions (ITIES) provides a unique analytical platform for the detection of ionic species of biological interest such as neurotransmitters and neuromodulators, especially those that are otherwise difficult to detect directly on a carbon electrode without electrode modification. We report the detection of acetylcholine, serotonin, and tryptamine on nanopipet electrode probes with sizes ranging from a radius of ?7 to 35 nm. The transfer of these analytes across a 1,2-dichloroethane/water interface was studied by cyclic voltammetry and amperometry. Well-defined sigmoidal voltammograms were observed on the nanopipet electrodes within the potential window of artificial seawater for acetylcholine and tryptamine. The half wave transfer potential, E1/2, of acetylcholine, tryptamine, and serotonin were found to be -0.11, -0.25, and -0.47 V vs E(1/2,TEA) (term is defined later in experimental), respectively. The detection was linear in the range of 0.25-6 mM for acetylcholine and of 0.5-10 mM for tryptamine in artificial seawater. Transfer of serotonin was linear in the range of 0.15-8 mM in LiCl solution. The limit of detection for serotonin in LiCl on a radius ?21 nm nanopipet electrode was 77 ?M, for acetylcholine on a radius ?7 nm nanopipet electrode was 205 ?M, and for tryptamine on a radius ?19 nm nanopipet electrode was 86 ?M. Nanopipet-supported ITIES probes have great potential to be used in nanometer spatial resolution measurements for the detection of neurotransmitters.
  • |Acetylcholine/chemistry/metabolism [MESH]
  • |Electrochemistry [MESH]
  • |Electrodes [MESH]
  • |Nanotechnology/instrumentation/*methods [MESH]
  • |Neurotransmitter Agents/*chemistry/metabolism [MESH]
  • |Oxidation-Reduction [MESH]
  • |Serotonin/chemistry/metabolism [MESH]


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