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Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Cold+Spring+Harb+Protoc 2015 ; 2015 (5): pdb.prot078287 Nephropedia Template TP
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Creation of Primary Cell Lines from Lineage-Labeled Mouse Models of Cancer #MMPMID25934932
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Cold Spring Harb Protoc 2015[]; 2015 (5): pdb.prot078287 PMID25934932show ga
Frequently, it is necessary to isolate pure populations of cancer cells for downstream assays, such as transcriptional analysis, signaling studies, and the creation of noncontaminated primary cell lines. Genetic lineage labeling with fluorescent reporter alleles allows for the identification of epithelial-derived cells within tumors. This protocol describes a method to isolate lineage-labeled pancreatic epithelial cells for ex vivo analysis, but it can be adapted for any type of lineage-labeled tumor.