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10.3109/02713683.2012.713152 http://scihub22266oqcxt.onion/10.3109/02713683.2012.713152 C4482234!4482234!22906079     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Curr+Eye+Res 2012 ; 37 (11): 1045-53 Nephropedia Template TP {{tp|p=22906079|t=2012. A Possible Role of Acrolein in Diabetic Retinopathy: Involvement of a VEGF/TGF? Signaling Pathway of the Retinal Pigment Epithelium in Hyperglycemia |pdf=|usr=}}{{22906079}} gab.com Text A Possible Role of Acrolein in Diabetic Retinopathy: Involvement of a VEGF/TGF Signaling Pathway of the Retinal Pigment Epithelium in Hyperglycemia JO: Curr Eye Res http://www.kidney.de/pget.php?&tw=1&pmid=22906079 #FOAvip Purpose: Acrolein has been implicated in retinal pigment epithelium (RPE) cell death, and has been associated with diabetic retinopathy. Our purpose was to investigate the potential effect of high glucose in influencing acrolein-mediated RPE cytokine production and cell death. We investigated the influence of the acrolein effect on ARPE-19 cells in high glucose conditions and quantified the release of transforming growth factor ? (TGF?1 and 2) and vascular endothelial growth factor (VEGF). We assessed the ability of N-benzylhydroxylamine(NBHA) as well as TGF? pathway inhibitors SIS3 and SB431542 to prevent this effect of acrolein on ARPE-19 cells. Materials and methods: Confluent ARPE-19 cells were treated with acrolein and/or NBHA in both 5.5 and 18.8 mM glucose conditions. Cells were also pretreated with SIS3, a specific inhibitor of the SMAD3 pathway, and SB431542, a specific inhibitor of TGF? signaling pathway, before treating them with acrolein. Viable cells were counted and ELISAs were performed to measure the cytokines TGF?1 and 2, and VEGF released into the conditioned media. Results: In ARPE-19 cells exposed to acrolein and hyperglycemia there was reduced cell viability and an increase in the cell media of VEGF, TGF?1, and TGF?2, which was reversed by NBHA. Acrolein/hyperglycemia-induced cell viability reduction and cytokine overproduction was also reduced by TGF? pathway blockade. Conclusions: We conclude that the effect of acrolein on the reduction of viability and VEGF increase by ARPE-19 cells in hyperglycemic media is conducted through the TGF? signaling pathway. Our results suggest that benefits of sequestering acrolein by NBHA and the blockage of the TGF? pathway by SB431542 and SIS3 offer suggestions as to potential useful pharmacological drug candidates for the prevention of diabetes-induced complications in the eye. Twit Text FOAVip A Possible Role of Acrolein in Diabetic Retinopathy: Involvement of a VEGF/TGF Signaling Pathway of the Retinal Pigment Epithelium in Hyperglycemia ????Curr Eye Res http://www.kidney.de/pget.php?&tw=1&pmid=22906079 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=22906079 #FOAvip English Wikipedia <ref>{{Cite pmid|22906079}}</ref> A Possible Role of Acrolein in Diabetic Retinopathy: Involvement of a VEGF/TGF? Signaling Pathway of the Retinal Pigment Epithelium in Hyperglycemia #MMPMID22906079 Grigsby J; Betts B; Vidro-Kotchan E; Culbert R; Tsin A Curr Eye Res 2012[Nov]; 37 (11): 1045-53 PMID22906079show ga Purpose: Acrolein has been implicated in retinal pigment epithelium (RPE) cell death, and has been associated with diabetic retinopathy. Our purpose was to investigate the potential effect of high glucose in influencing acrolein-mediated RPE cytokine production and cell death. We investigated the influence of the acrolein effect on ARPE-19 cells in high glucose conditions and quantified the release of transforming growth factor ? (TGF?1 and 2) and vascular endothelial growth factor (VEGF). We assessed the ability of N-benzylhydroxylamine(NBHA) as well as TGF? pathway inhibitors SIS3 and SB431542 to prevent this effect of acrolein on ARPE-19 cells. Materials and methods: Confluent ARPE-19 cells were treated with acrolein and/or NBHA in both 5.5 and 18.8 mM glucose conditions. Cells were also pretreated with SIS3, a specific inhibitor of the SMAD3 pathway, and SB431542, a specific inhibitor of TGF? signaling pathway, before treating them with acrolein. Viable cells were counted and ELISAs were performed to measure the cytokines TGF?1 and 2, and VEGF released into the conditioned media. Results: In ARPE-19 cells exposed to acrolein and hyperglycemia there was reduced cell viability and an increase in the cell media of VEGF, TGF?1, and TGF?2, which was reversed by NBHA. Acrolein/hyperglycemia-induced cell viability reduction and cytokine overproduction was also reduced by TGF? pathway blockade. Conclusions: We conclude that the effect of acrolein on the reduction of viability and VEGF increase by ARPE-19 cells in hyperglycemic media is conducted through the TGF? signaling pathway. Our results suggest that benefits of sequestering acrolein by NBHA and the blockage of the TGF? pathway by SB431542 and SIS3 offer suggestions as to potential useful pharmacological drug candidates for the prevention of diabetes-induced complications in the eye. äA Possible Role of Acrolein in Diabetic Retinopathy: Involvement of a (epmc).pdf DeepDyve Pubget Overpricing