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10.1093/nar/gkv386 http://scihub22266oqcxt.onion/10.1093/nar/gkv386 C4482092!4482092!25897131     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nucleic+Acids+Res 2015 ; 43 (9): 4721-32 Nephropedia Template TP {{tp|p=25897131|t=2015. Splicing of many human genes involves sites embedded within introns |pdf=|usr=}}{{25897131}} gab.com Text Splicing of many human genes involves sites embedded within introns JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=25897131 #FOAvip The conventional model for splicing involves excision of each intron in one piece; we demonstrate this inaccurately describes splicing in many human genes. First, after switching on transcription of SAMD4A, a gene with a 134 kb-long first intron, splicing joins the 3? end of exon 1 to successive points within intron 1 well before the acceptor site at exon 2 is made. Second, genome-wide analysis shows that >60% of active genes yield products generated by such intermediate intron splicing. These products are present at ?15% the levels of primary transcripts, are encoded by conserved sequences similar to those found at canonical acceptors, and marked by distinctive structural and epigenetic features. Finally, using targeted genome editing, we demonstrate that inhibiting the formation of these splicing intermediates affects efficient exon?exon splicing. These findings greatly expand the functional and regulatory complexity of the human transcriptome. Twit Text FOAVip Splicing of many human genes involves sites embedded within introns ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=25897131 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25897131 #FOAvip English Wikipedia <ref>{{Cite pmid|25897131}}</ref> Splicing of many human genes involves sites embedded within introns #MMPMID25897131 Kelly S; Georgomanolis T; Zirkel A; Diermeier S; O'Reilly D; Murphy S; Längst G; Cook PR; Papantonis A Nucleic Acids Res 2015[May]; 43 (9): 4721-32 PMID25897131show ga The conventional model for splicing involves excision of each intron in one piece; we demonstrate this inaccurately describes splicing in many human genes. First, after switching on transcription of SAMD4A, a gene with a 134 kb-long first intron, splicing joins the 3? end of exon 1 to successive points within intron 1 well before the acceptor site at exon 2 is made. Second, genome-wide analysis shows that >60% of active genes yield products generated by such intermediate intron splicing. These products are present at ?15% the levels of primary transcripts, are encoded by conserved sequences similar to those found at canonical acceptors, and marked by distinctive structural and epigenetic features. Finally, using targeted genome editing, we demonstrate that inhibiting the formation of these splicing intermediates affects efficient exon?exon splicing. These findings greatly expand the functional and regulatory complexity of the human transcriptome. äSplicing of many human genes involves sites embedded within introns (epmc).pdf DeepDyve Pubget Overpricing