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Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 BMC+Res+Notes 2015 ; 8 (ä): ä Nephropedia Template TP
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Surface plasmon resonance imaging of pathogens: the Yersinia pestis paradigm #MMPMID26105071
Huynh HTT; Gotthard G; Terras J; Aboudharam G; Drancourt M; Chabrière E
BMC Res Notes 2015[]; 8 (ä): ä PMID26105071show ga
Background: Yersinia pestis, causing deadly plague, is classified as a group A bioterrorism bacterium. Some recent DNA-based methods were used for detection of bioterrorism agents. Results: Y. pestis was used as a model organism to develop an immunosensor based on surface plasmon resonance imaging (SPRi) using monoclonal antibody against Y. pestis F1 antigen. The experimental approach included step-by-step detection of Y. pestis membrane proteins, lysed bacteria, intact bacteria, mock-infected powder and mock-infected clinical specimens. SPRi detected on average 106 intact Y. pestis organisms in buffer, in mock-infected powder and in a 1:4 mixture with HEL cells. Conclusions: This study offers the proof-of-concept of the SPRi-based detection of a human pathogen in both environmental and clinical specimens.