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10.1186/s13104-015-1236-3

http://scihub22266oqcxt.onion/10.1186/s13104-015-1236-3
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C4479326!4479326!26105071
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suck abstract from ncbi


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pmid26105071      BMC+Res+Notes 2015 ; 8 (ä): ä
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  • Surface plasmon resonance imaging of pathogens: the Yersinia pestis paradigm #MMPMID26105071
  • Huynh HTT; Gotthard G; Terras J; Aboudharam G; Drancourt M; Chabrière E
  • BMC Res Notes 2015[]; 8 (ä): ä PMID26105071show ga
  • Background: Yersinia pestis, causing deadly plague, is classified as a group A bioterrorism bacterium. Some recent DNA-based methods were used for detection of bioterrorism agents. Results: Y. pestis was used as a model organism to develop an immunosensor based on surface plasmon resonance imaging (SPRi) using monoclonal antibody against Y. pestis F1 antigen. The experimental approach included step-by-step detection of Y. pestis membrane proteins, lysed bacteria, intact bacteria, mock-infected powder and mock-infected clinical specimens. SPRi detected on average 106 intact Y. pestis organisms in buffer, in mock-infected powder and in a 1:4 mixture with HEL cells. Conclusions: This study offers the proof-of-concept of the SPRi-based detection of a human pathogen in both environmental and clinical specimens.
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