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10.1117/1.NPh.1.1.011013 http://scihub22266oqcxt.onion/10.1117/1.NPh.1.1.011013 C4478968!4478968 !26157969     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26157969 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Neurophotonics 2014 ; 1 (1 ): 011013 Nephropedia Template TP {{tp|p=26157969 |t=2014. Combined two-photon imaging, electrophysiological, and anatomical investigation of the human neocortex in vitro |pdf=|usr=}}{{26157969 }} gab.com Text Combined two-photon imaging, electrophysiological, and anatomical investigation of the human neocortex in vitro JO: Neurophotonics http://www.kidney.de/pget.php?&tw=1&pmid=26157969 #FOAvip Spontaneous synchronous population activity (SPA) can be detected by electrophysiological methods in cortical slices of epileptic patients, maintained in a physiological medium in vitro. In order to gain additional spatial information about the network mechanisms involved in the SPA generation, we combined electrophysiological studies with two-photon imaging. Neocortical slices prepared from postoperative tissue of epileptic and tumor patients were maintained in a dual perfusion chamber in a physiological incubation medium. SPA was recorded with a 24-channel extracellular linear microelectrode covering all neocortical layers. After identifying the electrophysiologically active regions of the slice, bolus loading of neuronal and glial markers was applied on the tissue. SPA-related [Formula: see text] transients were detected in a large population of neighboring neurons with two-photon microscopy, simultaneous with extracellular SPA and intracellular whole-cell patch-clamp recordings. The intracellularly recorded cells were filled for subsequent anatomy. The cells were reconstructed in three dimensions and examined with light- and transmission electron microscopy. Combining high spatial resolution two-photon [Formula: see text] imaging techniques and high temporal resolution extra- and intracellular electrophysiology with cellular anatomy may permit a deeper understanding of the structural and functional properties of the human neocortex. Twit Text FOAVip Combined two-photon imaging, electrophysiological, and anatomical investigation of the human neocortex in vitro ????Neurophotonics http://www.kidney.de/pget.php?&tw=1&pmid=26157969 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26157969 #FOAvip English Wikipedia <ref>{{Cite pmid|26157969 }}</ref> Combined two-photon imaging, electrophysiological, and anatomical investigation of the human neocortex in vitro #MMPMID26157969 Kerekes BP ; Tóth K ; Kaszás A ; Chiovini B ; Szadai Z ; Szalay G ; Pálfi D ; Bagó A ; Spitzer K ; Rózsa B ; Ulbert I ; Wittner L Neurophotonics 2014[Jul]; 1 (1 ): 011013 PMID26157969 show ga Spontaneous synchronous population activity (SPA) can be detected by electrophysiological methods in cortical slices of epileptic patients, maintained in a physiological medium in vitro. In order to gain additional spatial information about the network mechanisms involved in the SPA generation, we combined electrophysiological studies with two-photon imaging. Neocortical slices prepared from postoperative tissue of epileptic and tumor patients were maintained in a dual perfusion chamber in a physiological incubation medium. SPA was recorded with a 24-channel extracellular linear microelectrode covering all neocortical layers. After identifying the electrophysiologically active regions of the slice, bolus loading of neuronal and glial markers was applied on the tissue. SPA-related [Formula: see text] transients were detected in a large population of neighboring neurons with two-photon microscopy, simultaneous with extracellular SPA and intracellular whole-cell patch-clamp recordings. The intracellularly recorded cells were filled for subsequent anatomy. The cells were reconstructed in three dimensions and examined with light- and transmission electron microscopy. Combining high spatial resolution two-photon [Formula: see text] imaging techniques and high temporal resolution extra- and intracellular electrophysiology with cellular anatomy may permit a deeper understanding of the structural and functional properties of the human neocortex. ?Combined two-photon imaging, electrophysiological, and anatomical inve(epmc).pdf DeepDyve Pubget Overpricing