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10.1007/s00216-015-8711-5 http://scihub22266oqcxt.onion/10.1007/s00216-015-8711-5 C4477949!4477949!25925862     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Anal+Bioanal+Chem 2015 ; 407 (18): 5425-32 Nephropedia Template TP {{tp|p=25925862|t=2015. Molecular screening of cancer-derived exosomes by surface plasmon resonance spectroscopy |pdf=|usr=}}{{25925862}} gab.com Text Molecular screening of cancer-derived exosomes by surface plasmon resonance spectroscopy JO: Anal Bioanal Chem http://www.kidney.de/pget.php?&tw=1&pmid=25925862 #FOAvip We report on a generic method to detect and identify the molecular profile of exosomes either derived from cultured cell lines or isolated from biofluids. Exosomes are nanovesicles shed by cells into their microenvironment and carry the molecular identity of their mother cells. These vesicles are actively involved in intercellular communication under physiological conditions and ultimately in the spread of various diseases such as cancer. As they are accessible in most biofluids (e.g., blood, urine, or saliva), these biological entities are promising tools for cancer diagnostics, offering a non-invasive and remote access to the molecular state of the disease. The composition of exosomes derived from cancer cells depends on the sort and state of the tumor, requiring a screening of multiple antigens to fully characterize the disease. Here, we exploited the capacity of surface plasmon resonance biosensing to detect simultaneously multiple exosomal and cancer biomarkers on exosomes derived from breast cancer cells. We developed an immunosensor surface which provides efficient and specific capture of exosomes, together with their identification through their distinct molecular profiles. The successful analysis of blood samples demonstrated the suitability of our bioanalytical procedure for clinical use.Electronic supplementary material: The online version of this article (doi:10.1007/s00216-015-8711-5) contains supplementary material, which is available to authorized users. Twit Text FOAVip Molecular screening of cancer-derived exosomes by surface plasmon resonance spectroscopy ????Anal Bioanal Chem http://www.kidney.de/pget.php?&tw=1&pmid=25925862 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25925862 #FOAvip English Wikipedia <ref>{{Cite pmid|25925862}}</ref> Molecular screening of cancer-derived exosomes by surface plasmon resonance spectroscopy #MMPMID25925862 Grasso L; Wyss R; Weidenauer L; Thampi A; Demurtas D; Prudent M; Lion N; Vogel H Anal Bioanal Chem 2015[]; 407 (18): 5425-32 PMID25925862show ga We report on a generic method to detect and identify the molecular profile of exosomes either derived from cultured cell lines or isolated from biofluids. Exosomes are nanovesicles shed by cells into their microenvironment and carry the molecular identity of their mother cells. These vesicles are actively involved in intercellular communication under physiological conditions and ultimately in the spread of various diseases such as cancer. As they are accessible in most biofluids (e.g., blood, urine, or saliva), these biological entities are promising tools for cancer diagnostics, offering a non-invasive and remote access to the molecular state of the disease. The composition of exosomes derived from cancer cells depends on the sort and state of the tumor, requiring a screening of multiple antigens to fully characterize the disease. Here, we exploited the capacity of surface plasmon resonance biosensing to detect simultaneously multiple exosomal and cancer biomarkers on exosomes derived from breast cancer cells. We developed an immunosensor surface which provides efficient and specific capture of exosomes, together with their identification through their distinct molecular profiles. The successful analysis of blood samples demonstrated the suitability of our bioanalytical procedure for clinical use.Electronic supplementary material: The online version of this article (doi:10.1007/s00216-015-8711-5) contains supplementary material, which is available to authorized users. äMolecular screening of cancer-derived exosomes by surface plasmon reso(epmc).pdf DeepDyve Pubget Overpricing