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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 World+J+Gastroenterol
2015 ; 21
(23
): 7197-207
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Paeoniflorin inhibits human gastric carcinoma cell proliferation through
up-regulation of microRNA-124 and suppression of PI3K/Akt and STAT3 signaling
#MMPMID26109806
Zheng YB
; Xiao GC
; Tong SL
; Ding Y
; Wang QS
; Li SB
; Hao ZN
World J Gastroenterol
2015[Jun]; 21
(23
): 7197-207
PMID26109806
show ga
AIM: To examine the potential anti-tumor activity of paeoniflorin in the human
gastric carcinoma cell line MGC-803. METHODS: Cell viability and cytotoxic
effects in MGC-803 cells were analyzed using a
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate
dehydrogenase assay, respectively. Cell apoptosis of MGC-803 cells was measured
using flow cytometry, DAPI staining assay and caspase-3 activity assay.
Quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to
measure the expression of microRNA-124 (miR-124) in response to paeoniflorin. The
expression of phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt),
phospho-Akt (p-Akt) and phospho-signal transducer and activator of transcription
3 (p-STAT3) were also measured by quantitative RT-PCR and Western blot analysis
in normal, miR-124 and anti-miR-124 over-expressing MGC-803 cells, treated with
paeoniflorin. RESULTS: Paeoniflorin was found to inhibit MGC-803 cell viability
in a dose-dependent manner. Paeoniflorin treatment was associated with the
induction of apoptosis and caspase-3 activity in MGC-803 cells. Paeoniflorin
treatment significantly increased miR-124 levels and inhibited the expression of
PI3K, Akt, p-Akt and p-STAT3 in MGC-803 cells. Interestingly, the over-expression
of miR-124 inhibits PI3K/Akt and phospho-STAT3 expressions in MGC-803 cells. PI3K
agonist (IGF-1, 1 ?g/10 ?L) or over-expression of STAT3 reversed the effect of
paeoniflorin on the proliferation of MGC-803 cells. Over-expression of
anti-miR-124 in MGC-803 cells reversed paeoniflorin-induced up-regulation.
CONCLUSION: In summary, the in vitro data suggest that paeoniflorin is a
potential novel therapeutic agent against gastric carcinoma, which inhibits cell
viability and induces apoptosis through the up-regulation of miR-124 and
suppression of PI3K/Akt and STAT3 signaling.