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2015 ; 2015
(ä): 109353
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Development of a System and Method for Automated Isolation of Stromal Vascular
Fraction from Adipose Tissue Lipoaspirate
#MMPMID26167182
SundarRaj S
; Deshmukh A
; Priya N
; Krishnan VS
; Cherat M
; Majumdar AS
Stem Cells Int
2015[]; 2015
(ä): 109353
PMID26167182
show ga
Autologous fat grafting for soft tissue reconstruction is challenged by
unpredictable long-term graft survival. Fat derived stromal vascular fraction
(SVF) is gaining popularity in tissue reconstruction as SVF-enriched fat grafts
demonstrate improved engraftment. SVF also has potential in regenerative medicine
for remodeling of ischemic tissues by promoting angiogenesis. Since SVF cells do
not require culture expansion, attempts are being made to develop automated
devices to isolate SVF at the point of care. We report development of a closed,
automated system to process up to 500?mL lipoaspirate using cell size-dependent
filtration technology. The yield of SVF obtained by automated tissue digestion
and filtration (1.17 ± 0.5 × 10(5)?cells/gram) was equivalent to that obtained by
manual isolation (1.15 ± 0.3 × 10(5); p = 0.8), and the viability of the cells
isolated by both methods was greater than 90%. Cell composition included
CD34+CD31- adipose stromal cells, CD34+CD31+ endothelial progenitor cells, and
CD34-CD31+ endothelial cells, and their relative percentages were equivalent to
SVF isolated by the manual method. CFU-F capacity and expression of angiogenic
factors were also comparable with the manual method, establishing
proof-of-concept for fully automated SVF isolation, suitable for use in
reconstructive surgeries and regenerative medicine applications.
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