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10.1155/2015/747864 http://scihub22266oqcxt.onion/10.1155/2015/747864 C4475539!4475539!26137493     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biomed+Res+Int 2015 ; 2015 (ä): ä Nephropedia Template TP {{tp|p=26137493|t=2015. Isolation and Characterization of Human Lung Lymphatic Endothelial Cells |pdf=|usr=}}{{26137493}} gab.com Text Isolation and Characterization of Human Lung Lymphatic Endothelial Cells JO: Biomed Res Int http://www.kidney.de/pget.php?&tw=1&pmid=26137493 #FOAvip Characterization of lymphatic endothelial cells from the respiratory system may be crucial to investigate the role of the lymphatic system in the normal and diseased lung. We describe a simple and inexpensive method to harvest, isolate, and expand lymphatic endothelial cells from the human lung (HL-LECs). Fifty-five samples of healthy lung selected from patients undergoing lobectomy were studied. A two-step purification tool, based on paramagnetic sorting with monoclonal antibodies to CD31 and Podoplanin, was employed to select a pure population of HL-LECs. The purity of HL-LECs was assessed by morphologic criteria, immunocytochemistry, flow cytometry, and functional assays. Interestingly, these cells retain in vitro several receptor tyrosine kinases (RTKs) implicated in cell survival and proliferation. HL-LECs represent a clinically relevant cellular substrate to study lymphatic biology, lymphoangiogenesis, interaction with microbial agents, wound healing, and anticancer therapy. Twit Text FOAVip Isolation and Characterization of Human Lung Lymphatic Endothelial Cells ????Biomed Res Int http://www.kidney.de/pget.php?&tw=1&pmid=26137493 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26137493 #FOAvip English Wikipedia <ref>{{Cite pmid|26137493}}</ref> Isolation and Characterization of Human Lung Lymphatic Endothelial Cells #MMPMID26137493 Lorusso B; Falco A; Madeddu D; Frati C; Cavalli S; Graiani G; Gervasi A; Rinaldi L; Lagrasta C; Maselli D; Gnetti L; Silini EM; Quaini E; Ampollini L; Carbognani P; Quaini F Biomed Res Int 2015[]; 2015 (ä): ä PMID26137493show ga Characterization of lymphatic endothelial cells from the respiratory system may be crucial to investigate the role of the lymphatic system in the normal and diseased lung. We describe a simple and inexpensive method to harvest, isolate, and expand lymphatic endothelial cells from the human lung (HL-LECs). Fifty-five samples of healthy lung selected from patients undergoing lobectomy were studied. A two-step purification tool, based on paramagnetic sorting with monoclonal antibodies to CD31 and Podoplanin, was employed to select a pure population of HL-LECs. The purity of HL-LECs was assessed by morphologic criteria, immunocytochemistry, flow cytometry, and functional assays. Interestingly, these cells retain in vitro several receptor tyrosine kinases (RTKs) implicated in cell survival and proliferation. HL-LECs represent a clinically relevant cellular substrate to study lymphatic biology, lymphoangiogenesis, interaction with microbial agents, wound healing, and anticancer therapy. äIsolation and Characterization of Human Lung Lymphatic Endothelial Cel(epmc).pdf DeepDyve Pubget Overpricing